Apr 4, 2018 · The Golgi stain is a high contrast imaging method, as the cell appears in dark brown or black, and stands out clearly against the background. Another advantage of the Golgi stain is that it randomly stains a small population of cells, less than 1%.

The Golgi apparatus, long recognized for its roles in protein processing and vesicular trafficking, has recently been identified as a crucial contributor to innate immune signaling pathways. This review discusses our expanding understanding of the ...

In this chapter, we provide a detailed guide for the application of commonly used small molecules to study Golgi structure and function in vitro. Furthermore, we have curated a concise, validated list of endomembrane markers typically used in ...

Golgi's method is a silver staining technique that is used to visualize nervous tissue under light microscopy. The method was discovered by Camillo Golgi, an Italian physician and scientist, who published the first picture made with the technique in 1873. [1] .

Mar 25, 2022 · By combining reflective confocal imaging, two-photon imaging, and immunostaining, the cleared Golgi-Cox-stained tissue could be used for large-volume 3D imaging, immunostaining of specific cell types, multiple rounds of immunostaining, and the reconstruction of neural circuits.

Here, we describe the Golgi-Cox staining in such detail that should turn the staining into an easily feasible method for all scientists working in the neuroscience field. Keywords: Golgi, neuronal morphology, spines, dendrites, vibratome.

Jun 18, 2019 · Here, we describe a step by step novel Golgi-Cox staining method for cryosection, which is similar to the commercial Golgi staining kits; using this method will be easier to obtain a stable and personalized result in less time and with fewer reagents.

Apr 8, 2024 · Furthermore, the article delves into the Golgi apparatus's roles in key immune pathways, including the inflammasome-mediated activation of caspase-1, the cGAS-STING pathway, and TLR/RLR signaling. Overall, this review aims to provide insights into the multifunctional nature of the Golgi apparatus and its impact on innate immunity.

Mar 21, 2020 · Here, we review the innate immune signaling events that occur at or emanate from the Golgi and the microbial hijacking strategies that target the Golgi or Golgi-associated proteins to inhibit innate immune responses.

Jun 30, 2017 · By combining the Golgi-Cox staining with tissue clearing and immunostaining, Golgi-Cox-stained tissue could be used for large-volume 3D imaging, identification of cell types of...