Bacterial vector for expressing fusion proteins with a thrombin site. For other reading frames, use pGEX-4T-2 or pGEX-4T-3.

This page describes the characteristics of pGEX expression vectors used with Cytiva products.

The pGEX vectors are designed for inducible, high-level intracellular expression of genes or gene fragments as fusions with Schistosoma japonicum GST. Expression in E. coli yields tagged proteins with

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In these procedures, E. coli host cells are made competent and then transformed with either uncut pGEX DNA or recombinant pGEX DNA. If electroporation is used to transform the cells, see Appendix 3 (Electroporation). Otherwise, proceed as described below.

Bacterial vector for expressing GST fusions with a Factor Xa site. Sequence Author: GE Healthcare. Explore Over 2.7k Plasmids: pGEX Vectors (GE Healthcare) | More Plasmid Sets. Sticky ends from different BtgI sites may not be compatible. Sticky ends from different Bsu36I sites may not be compatible.

pGEX vectors are to be used for scientific investigation and research and for no other purpose whatsoever and a license for commercial use of the licensed products and the processes claimed in US patent 5,654,176 and equivalent patents and patent

Prepare fusion construct by inserting gene of interest into the multiple cloning site of pGEX-4T-1 using any one, or combination of unique restriction sites and transform into a host of choice such as E. coli BL21 (27154201).

PreScission Protease, Thrombin, or Factor Xa recognition sites for cleaving the desired protein from the fusion product. Thirteen pGEX vectors are available (see Figure). Nine of the vectors …

This page describes troubleshooting strategies for cloning the gene or gene fragment into a pGEX expression vector.