DNA topoisomerase 2-beta is an enzyme that in humans is encoded by the TOP2B gene. [5][6] This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription.

Nov 2, 2022 · Type II topoisomerases (TOP2) are conserved regulators of chromatin topology that catalyze reversible DNA double-strand breaks (DSBs) and are essential for maintaining genomic integrity in diverse dynamic processes such as transcription, replication, and cell division.

Dec 25, 2024 · TOP2B (DNA Topoisomerase II Beta) is a Protein Coding gene. Diseases associated with TOP2B include B-Cell Immunodeficiency, Distal Limb Anomalies, And Urogenital Malformations and Agammaglobulinemia 4, Autosomal Recessive. Among its related pathways are Transport of the SLBP independent Mature mRNA and Metabolism of proteins.

Nov 4, 2022 · Type II topoisomerases (TOP2) are conserved regulators of chromatin topology that catalyze reversible DNA double-strand breaks (DSBs) and are essential for maintaining genomic integrity in diverse dynamic processes such as transcription, replication, and cell division.

该基因编码一种 DNA 拓扑异构酶，一种在转录过程中控制和改变 DNA 拓扑状态的酶。 这种核酶参与染色体浓缩、染色单体分离以及 DNA 转录和复制过程中发生的扭转应力的缓解等过程。 它催化双链 DNA 的两条链的瞬时断裂和重新连接，使链彼此穿过，从而改变 DNA 的拓扑结构。 这种酶的两种形式可能是基因复制事件的产物。 编码这种形式的基因，β，位于 3 号染色体，而 alpha 形式位于 17 号染色体。 编码这种酶的基因作为几种抗癌剂的靶标，并且该基因的各种突变与 …

TOP2B is positioned to solve topological problems at diverse cis-regulatory elements and its occupancy is a highly ordered and prevalent feature of CTCF/cohesin binding sites that flank TADs. Data indicate that cortex involvement, lower World Health Organization grade and DNA topoisomerase II positivity were strong predictors for preoperative ...

Sep 14, 2018 · In 1987 the first report of a second type II isoform in mammalians was published by Fred Drake’s group at Smith Kline French (SKF); this isoform was called DNA topoisomerase IIβ (TOP2B) [1]. The purification of topoisomerase IIβ was from a murine P388 leukemia cell line selected for resistance to amsacrine [2].

Feb 27, 2024 · We trapped catalytically engaged topoisomerase IIβ (TOP2B) in covalent DNA cleavage complexes (TOP2Bccs) and mapped their positions genome-wide in cultured mouse cortical neurons. We report that TOP2Bcc distribution varies with both nucleosome and compartmental chromosome organization.

研究人员通过检测经典DDR通路中的 γ-H2AX信号，发现不同于外源诱导的DSBs，内源产生的DSBs并不激活DDR通路。 从修复方式来看， 外源诱导的DSBs既能够通过HR途径也能够通过NHEJ途径来进行修复，而内源产生的DSBs却主要通过NHEJ途径来进行修复 （XRCC4信号较强）。 前面说过，这种无修复模版的NHEJ途径保真性很差，容易造成包括染色体易位在内的染色体畸变。 事实上，研究人员也确实发现只有Pol II-pS5/TOP2B/XRCC4阳性的内含子在染色体易 …

Nov 2, 2022 · II 型拓扑异构酶 (TOP2) 是染色质拓扑结构的保守调节因子，可催化可逆的 DNA 双链断裂 (DSB)，对于在转录、复制和细胞分裂等多种动态过程中维持基因组完整性至关重要。 虽然受控的 TOP2 介导的 DSB 是解决 DNA 拓扑约束的优雅解决方案，但 DSB 也有助于导致癌症的染色体易位和突变的出现。 TOP2 酶作为一线化疗靶点的核心重要性是众所周知的。 然而，它们在癌症发展中的确切生物学功能和影响仍然知之甚少。 在这篇综述中，我们提供了 TOP2A 和 …