RNase II is the most active exoribonuclease in Escherichia coli cell extracts. Yet, its removal appears to have no deleterious effect on growing cells. Here, we show that RNase II is required for cell survival during prolonged stationary phase and upon starvation.

Ribonuclease II (RNase II) is a major exoribonuclease that intervenes in all of these fundamental processes; it can act independently or as a component of the exosome, an essential RNA-degrading multiprotein complex.

2006年9月7日 · RNase II is a ubiquitous exoribonuclease that processively hydrolyses RNA in the 3′ to 5′ direction, releasing 5′ monophosphates 1. The enzyme binds RNA and in the presence of...

RNase II, the 644 amino-acid product of the rnb gene, is the major hydrolytic exoribonuclease in E. coli. RNase II acts processively from the 3′ end of single-stranded RNA substrates and accounts for approximately 90% of the total exoribonucleolytic activity in cell extracts [6,11].

Here we review recent progresses in the understanding of the degradation mechanism of RNase II, based on mutational analysis and their characterization regarding catalysis and RNA affinity.

RNase II can also be stimulated by polyadenylation in vitro, but to a more limited extent . In order to resolve the paradoxical properties of RNase II and PNPase, we compared their abilities to degrade short synthetic RNA substrates containing a …

RNase II from Escherichia coli is the prototype of this family, which also includes bacterial RNase R and eukaryotic Rrp44/Dis3 proteins. These proteins present a similar modular organization with cold shock domains (CSD) at the N-terminal region involved in RNA binding, a central RNB domain responsible for catalysis, and a C-terminal S1 domain ...

RNase II is a key exoribonuclease involved in the maturation, turnover, and quality control of RNA. RNase II homologues are components of the exosome, a complex of exoribonucleases. The structure of RNase II unraveled crucial aspects of the mechanism of RNA degradation.

2020年4月17日 · To our knowledge, Anabaena RNase II is an unusual ribonuclease that interacts with the catalytic domain of RNase E, and it may represent a new type of RNA degradosome and a novel mechanism for regulating the activity of the RNA degradosome.

RNase II and RNase R are the two E. coli exoribonucleases that belong to the RNase II super family of enzymes. They degrade RNA hydrolytically in the 3' to 5' direction in a processive and sequence independent manner.