Ribonuclease E is a bacterial ribonuclease that participates in the processing of ribosomal RNA (9S to 5S rRNA) and the chemical degradation of bulk cellular RNA. RNase E was suggested …

Dec 14, 2012 · RNase E is a central player in both stable-RNA processing and mRNA decay. It initiates the processing of about two-thirds of all pre-tRNAs and the decay of most mRNAs. It …

The activity of RNase E can be directed and modulated by signals provided through regulatory RNAs that guide the enzyme to specific transcripts that are to be silenced. Early in its …

Jan 13, 2025 · RNase E is a pivotal enzyme in bacterial RNA metabolism, playing a key role in the processing and degradation of RNA. Its function is essential for maintaining cellular …

RNase E, the main component of RNA degradosome in Escherichia coli, plays an important role in mRNA degradation and RNA transcript processing (4, 5). It recognizes and cuts single …

As noted above, RNase E serves many important functions in E. coli cells, acting to process or degrade essentially every type of RNA molecule. RNase E also is an essential enzyme, …

Oct 15, 1998 · Ribonuclease (RNase) E (ref. 1), a single-strand-specific endonuclease 2, 3, 4 that is found in a multi-enzyme complex known as the ‘degradosome’ 5, 6, 7, initiates the …

The essential endoribonuclease of E. coli is named RNase E. RNase E-related enzymes are present in many bacterial species and are thought to be the primary decay-initiating enzyme in …

Here we show using RNA-seq analyses of ribonuclease-deficient strains in vivo and a 5′-sensor mutant of RNase E in vitro that, contrary to current models, 5′-monophosphate-independent, …

Nov 1, 2019 · Here, using recombinantly expressed and purified proteins, site-directed mutagenesis, and RNA cleavage and protein cross-linking assays, we investigated Hfq …

3 days ago · Purified RNase E 1-529-Flag, RNase E 1-588-Flag, RNase E 572-1057-Flag, RNase E 572-1057_NDPRmut - Flag, or RNase E 572-1057_AR1mut - Flag fusion proteins (20-25 µg …

Mar 31, 2021 · RNase Y and RNase E are disparate endoribonucleases that govern global mRNA turnover/processing in the two evolutionary distant bacteria Bacillus subtilis and Escherichia …

In this Review, I summarize the major roles of RNase E in RNA processing and decay and discuss the various mechanisms that regulate its activity. I also propose a new model to …

RNase E enzyme from E. coli cells, and herein describe the isolation and some characterization of this enzyme and the reactions which lead from 9 S to p5.

RNase-E-Dependent sRNA Biogenesis and Maturation in Bacteria. We identify RNase E as a key factor both for the biogenesis of many 3′ UTR-derived sRNAs and for the maturation of active …

3 days ago · f A549 cells were treated with RNase R (3 U of RNase R/μg RNA for 10 min), and qPCR was performed to measure the expression levels of circPLCE1 and PLCE1 mRNAs. ...

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RNase E is an essential endoribonuclease involved in RNA processing and mRNA degradation. The N-terminal half of the protein encompasses the catalytic domain; the C-terminal half is the …

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