2012年12月14日 · RNase E is a central player in both stable-RNA processing and mRNA decay. It initiates the processing of about two-thirds of all pre-tRNAs and the decay of most mRNAs. It also...

RNase E, the main component of RNA degradosome in Escherichia coli, plays an important role in mRNA degradation and RNA transcript processing (4, 5). It recognizes and cuts single-stranded RNA with numerous AU bases, thereby cleaving mRNA …

The activity of RNase E can be directed and modulated by signals provided through regulatory RNAs that guide the enzyme to specific transcripts that are to be silenced. Early in its evolutionary history, RNase E acquired a natively unfolded appendage …

2025年1月13日 · RNase E is a pivotal enzyme in bacterial RNA metabolism, playing a key role in the processing and degradation of RNA. Its function is essential for maintaining cellular homeostasis by regulating RNA levels and ensuring proper gene expression.

Here we show using RNA-seq analyses of ribonuclease-deficient strains in vivo and a 5′-sensor mutant of RNase E in vitro that, contrary to current models, 5′-monophosphate-independent, ‘direct entry’ cleavage is a major pathway for degrading and processing RNA.

Many of the mechanisms that enable efficient RNA processing and degradation in bacteria have analogous processes in organisms of other domains of life, including metazoans. RNase E has been a key paradigm in understanding the complexity of RNA-mediated regulation and metabolism in bacteria.

As noted above, RNase E serves many important functions in E. coli cells, acting to process or degrade essentially every type of RNA molecule. RNase E also is an essential enzyme, indicating that no other RNase is able to take over at least one of its many functions in its absence.

The essential endoribonuclease of E. coli is named RNase E. RNase E-related enzymes are present in many bacterial species and are thought to be the primary decay-initiating enzyme in these organisms. RNase E is a 5′-end-dependent endonuclease, that is, it binds to the 5′ end of an mRNA and then locates a target site at which it cleaves ...

In this Review, I summarize the major roles of RNase E in RNA processing and decay and discuss the various mechanisms that regulate its activity. I also propose a new model to rationalize the mechanism of RNase E action in the context of its localization in the bacterial cell.

2019年11月1日 · Here, using recombinantly expressed and purified proteins, site-directed mutagenesis, and RNA cleavage and protein cross-linking assays, we investigated Hfq-independent RNA decay by RNase E. Exploring its RNA substrate preferences in the absence of Hfq, we observed that RNase E preferentially cleaves AU-rich sites of single-stranded regions …