The expected band sizes for pTrc99A and λZAPII DNA after digestion with Hind III are as follows:

With respect to the pTrc99A/topAcysB expression plasmid, what is the immediate consequence of IPTG binding to the above molecule? 2 points Synthesis of B-galactosidase Binding of DNA …

(2 points) Submit Answer Tries 0/3 Assuming that you placed 200 ng of vector DNA (pTrc99A) in your original reaction tube (Steps 2 & 3, Expt. 4B) and removed 16 pl for electrophoresis (Step …

What is the purpose of growing culture of XL-1-Blue containing pTrc99A in the presence of ampicillin? Your solution’s ready to go! Our expert help has broken down your problem into an …

Question: Exp. 6B: Transformants of E. coli DM700 (topA-cysB) containing pTrc99A with an insert of the topA-cysB region of the E. coli chromosome were obtained by selection for growth in the …

Assuming a plasmid copy number of 180 plasmids per cell, and a 100% recovery of plasmid DNA from 3.00 mL of the cells, what is the theoretical yield of plasmid DNA in micrograms (ug)? 2 …

Answer to Was pTrc99A and zapii/topA extracted? Give reasons

Question: Exp. 4B: In this experiment, the topA gene and the adjacent cysB gene from the segment of E. coli DNA contained in λZAPII/topAcysB will be inserted into the vector pTrc99A. …

You add 7ng of pTrc99A-topAcysB (7981 bp) to the PCR reaction to serve as a template. After 33 cycles of PCR, what is the theoretical yield in number of DNA fragments? (6.02*10 …

Question: You measure the OD600 of your culture of chloramphenicol-treated XL1-Blue/pTrc99A cells as described in Step 1 of Experiment 3A and obtain a value of 0.376. Assuming a …