The expected band sizes for pTrc99A and λZAPII DNA after digestion with Hind III are as follows:

With respect to the pTrc99A/topAcysB expression plasmid, what is the immediate consequence of IPTG binding to the above molecule? 2 points Synthesis of B-galactosidase Binding of DNA polymerase to the lactose promoter Repression of the lactose operator Replication of pTrc99A Binding of RNA polymerase to Ptrc Incorrect.

(2 points) Submit Answer Tries 0/3 Assuming that you placed 200 ng of vector DNA (pTrc99A) in your original reaction tube (Steps 2 & 3, Expt. 4B) and removed 16 pl for electrophoresis (Step 4, Expt. 4B), what mass of vector DNA, to the nearest nanogram, would you expect to see in Lane 3 of the gel in Expt. 5B?(1 point) 375 ng Submit Answer ...

What is the purpose of growing culture of XL-1-Blue containing pTrc99A in the presence of ampicillin? Your solution’s ready to go! Our expert help has broken down your problem into an easy-to-learn solution you can count on.

Question: Exp. 6B: Transformants of E. coli DM700 (topA-cysB) containing pTrc99A with an insert of the topA-cysB region of the E. coli chromosome were obtained by selection for growth in the absence of cysteine. Such cells should also display ampicillin resistance, owing to expression of the ampR gene of the plasmid.

Assuming a plasmid copy number of 180 plasmids per cell, and a 100% recovery of plasmid DNA from 3.00 mL of the cells, what is the theoretical yield of plasmid DNA in micrograms (ug)? 2 points (Note: the size of pTrc99A is 4176 base pairs and the average molecular weight of double-stranded DNA is 660 g/mole of base pairs; report your answer ...

Answer to Was pTrc99A and zapii/topA extracted? Give reasons

Question: Exp. 4B: In this experiment, the topA gene and the adjacent cysB gene from the segment of E. coli DNA contained in λZAPII/topAcysB will be inserted into the vector pTrc99A. λZAPII/topAcysB DNA will be cleaved with SacI and XbaI restriction enzymes.

You add 7ng of pTrc99A-topAcysB (7981 bp) to the PCR reaction to serve as a template. After 33 cycles of PCR, what is the theoretical yield in number of DNA fragments? (6.02*10 1023bp/molbp,660 g/molbp ) Round your answer to 3sig figs. (2 points) Tries 0/5 What is the theoretical yield in ug? Round your answer to 3 sig figs. (2 points) Tries 0/5

Question: You measure the OD600 of your culture of chloramphenicol-treated XL1-Blue/pTrc99A cells as described in Step 1 of Experiment 3A and obtain a value of 0.376. Assuming a plasmid copy number of 630 plasmids per cell, and a 100% recovery of plasmid DNA from 3.00 mL of the cells, what is the theoretical yield of plasmid DNA in micrograms ...