The patch clamp technique is a laboratory technique in electrophysiology used to study ionic currents in individual isolated living cells, tissue sections, or patches of cell membrane.

2023年5月4日 · This article gives an introduction to the patch-clamp technique and how it is used to study the physiology of ion channels for neuroscience and other life-science fields.

2018年10月23日 · The patch clamp is a laboratory technique for studying currents in living cells. It is usually carried out by applying a voltage across the cell membrane and measuring the...

The patch clamp method is a refined electrophysiological technique that can directly measure the membrane potential and/or the amount of current passing across the cell membrane.

The patch-clamp technique represents an electrophysiology type of method. This is one of several insightful approaches with five major configurations, namely a loose patch, cell-attached (also known as on-cell), whole-cell, inside-out, and outside-out modes.

The patch clamp technique is widely used to investigate cellular behavior of excitatory neurons at the single-cell level in vitro. Initially, patch clamping — applying a fixed voltage and measuring the current across the cell membrane using a pipette — allowed researchers to study electrophysiology in one single cell at a time.

In vivo whole-cell patch-clamp techniques have been used to observe spontaneous or sensory-evoked subthreshold dynamics in various brain regions, such as the neocortex, hippocampus, thalamus, amygdala, and others.

The most common method used to assess ion-channel function is known as the patch-clamp electrophysiological technique that was developed in the 1970s by Nobel Prize Laureates Erwin Neher and Bert Sakmann.

2012年3月2日 · The patch-clamp technique, used under the voltage-clamp mode, is the golden standard in the study of ion channels and measures the ion flux or ionic currents through the open channels. Recording of ion channel currents can be performed in various configurations.

The Patch-clamp technique is a versatile electrophysiological tool for understanding ion channel behavior. Every cell expresses ion channels, but the most common cells to study with patch-clamp techniques include neurons, muscle fibers, cardiomyocytes, and oocytes overexpressing single ion channels.