LX-2 was generated by immmortalization of primary human hepatic stellate cells with the SV40 large T antigen followed by selective culture of early passaged cells in low serum media conditions. Immortalized LX-2 was established by Xu et al to overcome issues of culture variability and to provide a stable and unlimited source of human hepatic ...

lx-2 ，人 肝星形细胞 。 肝星形细胞在慢性酒精中毒、 乙型和丙型肝炎 、 脂肪性肝病 、肥胖和糖尿病等疾病中扮演重要角色，激活后为肌成纤维细胞，是负责肝纤维化的主要细胞类型。 该细胞系名称来源于原始论文第一作者的名字，是2004年取hsc细胞转染 sv40大t抗原 而建立的永生化细胞 …

Recently, LX-2 cells were shown to be migratory due to increased MMP-2 activity from cytokine stimulation. 38 Increasing evidence also links matrix degradation directly to apoptosis in stellate cells, 39 and the unique capacity of LX-2 cells to withstand serum deprivation not only provides a means to assay MMP-2 by zymography without the ...

Representative fluorescent imaging of ACTA2 filaments in LX-2 cells. Cells shown stained with anti-ACTA and Alexa Fluor 488 secondary antibody. A non-treated or B treated with transforming growth factor-β1 (TGF-β1). As can be seen, TGF-β1 increased the appearance of organised ACTA2 filaments. Scale bar = 100 μm

Oct 8, 2013 · The human hepatic cell line LX-2 has been described as tool to study mechanisms of hepatic fibrogenesis and the testing of antifibrotic compounds. It was originally generated by immortalisation with the Simian Vacuolating Virus 40 (SV40) transforming (T) antigen and subsequent propagation in low serum conditions.

Hepatic stellate cells are a major cell type responsible for liver -like cells in diseases such as chronic alcoholism, hepatitis B and C, fatty liver disease, obesity and diabetes. There is an increasing need for renewable cell culture models that faithfully recapitulate their in vivo phenotype, particularly for human studies.

Human hepatic stellate cell line (LX-2) exhibits characteristics of bone marrow-derived mesenchymal stem cells. Exp. Mol. Pathol. 91:664-672(2011) PubMed=22694338; DOI=10.1021/pr3000927; PMCID=PMC3411196 Rashid S.T., Humphries J.D., Byron A., Dhar A., Askari J.A., Selley J.N., Knight D., Goldin R.D., Thursz M., Humphries M.J.

LX-2 cells are used to examine the cellular and molecular mechanisms underlying liver fibrosis, including the role of stellate cells in extracellular matrix deposition and the modulation of these processes by therapeutic agents.

Dec 1, 2011 · To detect the mesenchymal origin of LX-2 cells, we compared the expression levels of surface markers and genes related to mesenchymal stem cells, pericytes and endothelial progenitor cells, and we analyzed the growth rates of LX-2, HBMS and primary BM-MSCs.

Oct 25, 2018 · Note higher E-cadherin and lower Vimentin expression levels in tumour cells produced by injecting MHCC97H-shTGM2 cells and LX2 cells into nude mice, compared with counterpart injection of MHCC97H ...