In molecular biology lin-4 is a microRNA (miRNA) that was identified from a study of developmental timing in the nematode Caenorhabditis elegans. [1] [2] It was the first to be discovered of the miRNAs, a class of non-coding RNAs involved in gene regulation.

1993年12月3日 · lin-4 is essential for the normal temporal control of diverse postembryonic developmental events in C. elegans. lin-4 acts by negatively regulating the level of LIN-14 protein, creating a temporal decrease in LIN-14 protein starting in the first larval stage (L1).

Our fundamental understanding of miRNA mechanisms arose from studying lin-4 in the simple nematode Caenorhabditis elegans (C. elegans).

1993年12月3日 · lin-4 is essential for the normal temporal control of diverse postembryonic developmental events in C. elegans. lin-4 acts by negatively regulating the level of LIN-14 protein, creating a temporal decrease in LIN-14 protein starting in the first larval stage (L1).

In this study, we characterized the expression of endogenous lin-4 and found that temporally regulated independent transcripts, but not constitutive lin-4 containing RNAs derived from an overlapping gene, are processed to mature lin-4 miRNA.

1993年12月3日 · lin-4 is essential for the normal temporal control of diverse postembryonic developmental events in C. elegans. lin-4 acts by negatively regulating the level of LIN-14 protein, creating a temporal decrease in LIN-14 protein starting in the first larval stage (L1).

Here, we monitor the dynamics of lin-14 mRNA and protein as well as lin-4 miRNA levels in finely staged animals during early larval development.

2012年6月12日 · Our results show that lin-4 microRNA acts as a potent and specific negative regulator of UNC-6–mediated attraction without affecting SLT-1–mediated repulsion, and we provide evidence that lin-4 microRNA suppresses production of the transcription factor LIN-14 in AVM neurons to inhibit attraction of their axons to UNC-6.

1999年12月15日 · lin-4 encodes a small RNA that is complementary to sequences in the 3′ untranslated region (UTR) of lin-14 mRNA and that acts to developmentally repress the accumulation of LIN-14 protein.

lin-4 is essential for the normal temporal control of diverse postembryonic developmental events in C. elegans. lin-4 acts by negatively regulating the level of LIN-14 protein, creating a temporal decrease in LIN-14 protein starting in the first larval stage (L1).