Ku80 is a protein that, in humans, is encoded by the XRCC5 gene. [5] Together, Ku70 and Ku80 make up the Ku heterodimer, which binds to DNA double-strand break ends and is required for the non-homologous end joining (NHEJ) pathway of DNA repair.

Ku80 is important for the repair of DNA double-strand breaks by the nonhomologous end-joining protein, Ku70. The Ku80–Ku70 heterodimer (Ku) binds to DNA ends, nicks, gaps, and hairpins.

Understanding the molecular basis of c-NHEJ has major outcomes in several fields, including radiobiology, cancer therapy, immune disease, and genome editing. The heterodimer Ku70/80 (Ku) is a central actor of the c-NHEJ as it rapidly recognizes broken DNA ends in the cell and protects them from nuclease activity.

2024年2月1日 · Based on our findings, we suggest that the high efficiency of Ku80 knockout in human HCT116 cells makes it a promising model for characterizing SVs in the context of 3D chromatin structure and studying the alternative-end joining (Alt …

Eukaryotic Ku is a heterodimer of two polypeptides, Ku70 (XRCC6) and Ku80 (XRCC5), so named because the molecular weight of the human Ku proteins is around 70 kDa and 80 kDa. The two Ku subunits form a basket-shaped structure that threads onto the DNA end. [1]

Ku70 or Ku80, we performed in vitro pull-down experiments us-ing purified recombinant His-tagged green fluorescent protein (His-GFP), His-cGAS-GFP, His-Ku80, or His-Ku70 from Escher-ichia coli. Using anti-GFP beads, we found that Ku80 and Ku70 were both directly pulled down by cGAS-GFP but not by GFP (Figures 1C and 1D).

2004年5月1日 · Ku is a heterodimer composed of two subunits — Ku70 and Ku80 — that binds to DNA ends in a sequence-independent manner. From crystallographic studies, we know that it is shaped like an asymmetric...

Our results suggest that the C-terminal region of Ku80 is dispensable for interaction with Ku70 and for DNA end binding but is required for optimal DNA-PK activity.

Previously we showed that control and ku80 −/− mice presented with external signs of inflammation as they aged; however, these signs were observed earlier in ku80 −/− mutant mice . These signs typically include suppurative conjunctivitis with …

2016年12月20日 · Studies showed that the Ku70/80 heterodimer is a key-player in the double-stranded DNA repair pathway nonhomologous end-joining (NHEJ) and binds to DNA termini with high affinity, where it helps to protect DNA ends from degradation and to recruit other NHEJ factors required for repair.