Kompetitive allele specific PCR (KASP) is a homogenous, fluorescence-based genotyping variant of polymerase chain reaction. It is based on allele-specific oligo extension and fluorescence resonance energy transfer for signal generation.

KASP genotyping assays are based on competitive allele-specific PCR and enable bi-allelic scoring of single nucleotide polymorphisms (SNPs) and insertions and deletions (Indels) at specific loci. KASP has been cited in thousands of peer reviewed scientific publications for hundreds of species with millions of KASP markers identified.

The KASP genotyping assay utilizes a unique form of competitive allele-specific PCR combined with a novel, homogeneous, fluorescence-based reporting system for the identification and measurement of genetic variation occurring at the nucleotide level to detect single nucleotide polymorphisms (SNPs) o …

2023年6月30日 · Molecular marker-assisted breeding techniques enable plant breeders to quickly and accurately identify and enhance the traits of interest. KASP, or competitive allele-specific PCR, is the most recent development in the field of molecular markers-aided breeding technology.

The KASP markers Xicsk8 and Xicsk13 (Figure 4A,B) and SSR markers Xicscx497 and Xicsx538 (Figure 6A,B) produced clear banding patterns and were able to differentiate individuals of the mapping population with distinct phenotypes.

2024年4月15日 · KASP is an allele-specific oligo extension-based PCR assay that uses fluorescence resonance energy transfer (FRET) to detect genetic variations such as SNPs and insertions/deletions (InDels) at a specific locus.

The EiB low-density genotyping service is based on KASP markers. Kompetitive Allele Specific PCR (KASP) is a simplified fluorescence-based methodology to genotype specific polymorphisms or INDELS.

Kompetitive Allele Specific PCR (KASP™) assays enable accurate, bi-allelic discrimination of SNPs and indels. Unmatched quality, reliability and credibility have established KASP as a foundational SNP genotyping chemistry for agrigenomics.

Anchoring allows KASP assays to genotype SNPs or indels in DNA samples that are known to contain multiple DNA sequences that are highly similar to the sequence surrounding the polymorphism of interest.

KASP markers have obvious advantages over other DNA molecular markers. Previous studies have shown that KASP assays are 45 times faster than gel-based PCR markers . For the development of markers to be used for high-throughput genotyping, the SNPs significantly associated with protein quality traits identified in the GWAS were selected, and ...