Cre-ERT2在无Tamoxifen诱导的情况下，在细胞质内处于无活性状态；当Tamoxifen诱导后，Tamoxifen的代谢产物4-OHT（雌激素类似物）与ERT结合，可使Cre-ERT2进核发挥Cre重组酶活性。 常见的基因工程小鼠可以分为两种命名方式，包括基因定点修饰的小鼠命名，比如：敲除、敲入、点突变等等，和随机转基因的小鼠命名。

2011年1月27日 · We hypothesize that HFE modulates erythropoiesis by affecting dietary iron absorption and erythroid iron intake. To investigate this, we used Hfe -KO mice in conditions of altered dietary iron and erythropoiesis. We show that Hfe -KO mice can overcome phlebotomy-induced anemia more rapidly than wild-type mice (even when iron loaded).

Our data provide evidence that both wt- and Hfe-KO-derived chondrocytes, when exposed to 50 μM iron, develop characteristics of an OA-related phenotype, such as an increased expression of metalloproteases, a decreased extracellular matrix production, and a …

Results: Hfe-KO mice showed a systemic iron overload and an increased iron accumulation in the knee synovial membrane following surgery. The histological OA score was significantly higher in the Hfe-KO mice at 8 weeks after surgery.

In summary, we have developed a new tool for gene therapy that increases expression of Hamp in the liver, partially reversing the hemochromatosis phenotype. Finally, studies are in progress to identify and optimize the conditions needed to completely prevent hemochromatosis in …

Our data provide evidence that both wt- and Hfe -KO-derived chondrocytes, when exposed to 50 μM iron, develop characteristics of an OA-related phenotype, such as an increased expression of metalloproteases, a decreased extracellular matrix production, and a …

携带突变Hfe基因的鼠与Cre敲除品系杂交后，后代保留了新霉素抗性基因座，但缺失了编码α-2、α-3和跨膜区的3-5个外显子。RT-PCR结果显示异常的转录本，它将2号外显子与6号外显子连接在一起。对肝脏提取物进行免疫印迹实验表明，突变基因座不产生任何蛋白质。

江苏集萃药康生物科技股份有限公司成立于2017年，是一家专业从事实验动物小鼠模型的研发、生产、销售及相关技术服务的高新技术企业。

2018年2月14日 · We found increased neuronal cell death, vascular alterations and loss of retinal barrier integrity in diabetic HFE KO mice compared to diabetic wildtype mice. Diabetic HFE KO mouse retinas also...

We hypothesize that HFE modulates erythropoiesis by affecting dietary iron absorption and erythroid iron intake. To investigate this, we used Hfe -KO mice in conditions of altered dietary iron and erythropoiesis. We show that Hfe -KO mice can overcome phlebotomy-induced anemia more rapidly than wild-type mice (even when iron loaded).