GCaMP is a genetically encoded calcium indicator (GECI) initially developed in 2001 by Junichi Nakai. [1] . It is a synthetic fusion of green fluorescent protein (GFP), calmodulin (CaM), and …

Jun 22, 2022 · GCaMP6f是一种基因编码的钙离子指示剂，常用于监测神经元活动。相较于GCaMP3，GCaMP6f的灵敏度提高了10倍，动力学快2倍，特别适用于检测快速钙信号变化 …

GCaMP6是一种超灵敏的荧光蛋白，可以特异高效地反映细胞内游离Ca2+浓度的变化。GCaMP6荧光蛋白可以作为一种超敏Ca2+探针，用于研究生理和病理情况下外界因素对活细 …

Mar 15, 2023 · The GCaMP6 (ref. 5) and jGCaMP7 (ref. 6) sensors enable detection of single action potentials (APs) under favourable conditions and are often used to monitor the activity …

Jul 17, 2013 · Fluorescent calcium sensors are widely used to image neural activity. Using structure-based mutagenesis and neuron-based screening, we developed a family of …

Jul 28, 2015 · GCamp是 钙指示剂，为啥结合谷氨酸会发光。 GCamp由 CaM，cpEGFP和M13组成，钙离子存在时与CaM结合使得CaM与M13发生构象变化，这样的变化也牵扯cpEGFP构象 …

基于结构的诱变和神经元的筛选，作者开发了一系列超灵敏钙离子指示剂(gcamp6)，在培养的神经元、斑马鱼、苍蝇和老鼠体内，这种指示剂的性能优于其他指示剂。

Jul 18, 2013 · Fluorescent calcium sensors are widely used to image neural activity. Using structure-based mutagenesis and neuron-based screening, we developed a family of …

特点： GcaMP6 是一种超灵敏的荧光蛋白，可以特异高效地反映细胞内游离Ca2+浓度的变化，在活体下，对单个动作电位诱发的钙信号，GCaMP6 比GCaMP3强10 倍，动力学快2 倍。

GCaMP6 expression can be selectively targeted to neuronal populations of interest, and potentially thousands of neurons can be captured within a single frame, allowing the neuron-by …