Ferroportin-1 plays an important role in neural tube closure and forebrain patterning. [17] Mouse embryos lacking the Slc40a1 gene are aborted before gastrulation occurs, suggesting that the Fpn1 protein encoded is necessary and essential for normal embryonic development. [15]

Ferroportin 1 (FPN1) is the only protein in mammalian cells that transports iron to the outside [35]. In neurons, amyloid precursor protein (APP) connects with FPN1 and stabilizes the expression level of FPN1, and APP is considered to be a ferrooxidase, which can oxidize Fe2 + to Fe3 + and export it out of cells [36].

Ferroportin Disease (FD) is an autosomal dominant hereditary iron loading disorder associated with heterozygote mutations of the ferroportin-1 (<i>FPN</i>) gene. It represents one of the commonest causes of genetic hyperferritinemia, regardless of ethnicity. FPN1 transfers iron from the intestine, m …

Aug 19, 2019 · Recent findings demonstrate that aberrant downregulation of the iron-exporter protein, ferroportin (FPN1), is associated with poor prognosis and osteoclast differentiation in multiple...

Aug 12, 2016 · FPN1 is important for the export of iron across the basolateral membrane of absorptive enterocytes and across the plasma membrane of macrophages. The expression of FPN1 is regulated by hepcidin, which binds to FPN1 and then induces its degradation.

Nov 25, 2010 · FPN1 mRNA levels were assayed by semiquantitative RT-PCR, and the data were normalized to actin mRNA. Cobalt, copper, iron, and zinc induced the expression of FPN1 mRNA in a dose-dependent manner (Figure 1 A). Cadmium induced FPN1 …

Ferroportin 1 (FPN1; SLC40A1) is the sole iron exporter from mammalian cells and is expressed in the duodenum and macrophages. In the present study, we show that FPN1 mRNA levels in the mouse macrophage cell line RAW264.7 are synergistically induced by treatment with live or γ-irradiated M. tuberculosis and IFN-γ.

FPN1 transfers iron from the intestine, macrophages and placenta into the bloodstream. In FD, loss-of-function mutations of FPN1 limit but do not impair iron export in enterocytes, but they do severely affect iron transfer in macrophages.

Mar 22, 2017 · This study examined FPN1 expression in human macrophages, the cells that are primarily responsible on a daily basis for plasma iron turnover and are central in the pathogenesis of ferroportin disease (FD), the disease attributed to lack‐of‐function FPN1 mutations.

Iron regulatory protein (IRPs)/iron-responsive element (IRE) is the classical system that regulates the expression of iron homeostasis proteins which including the transferrin receptor 1 (TfR1), the divalent metal transporter 1 (DMT1), ferritin and ferroportin …

The coordination between iron entry into plasma, iron utilization, and iron storage is accomplished by the interaction of the peptide hormone hepcidin and the iron transporter, ferroportin (FPN) encoded by FPN1 (SLC40A1). Hepcidin is a member …

Jul 1, 2023 · In this article, we discuss the current understanding of Fpn1 expression in the brain and cell lines of rats, mice and humans, with emphasis on the potential involvement of reduced Fpn1 in brain iron enhancement in patients with AD, PD and other NDs.

Ferroportin1 (FPN1) is the only mammalian protein associated with iron release and thus plays a vital role in iron homeostasis, while nuclear factor E2-related factor 2 (NRF2) controls the transcription of FPN1.

Sep 8, 2011 · Here, our results with both transiently transfected FPN1 in erythroblast cell lines and endogenous FPN1 in primary fetal liver erythroblasts showed that FPN1 trafficks to the plasma membrane, the subcellular location required …

This study examined FPN1 expression in human macrophages, the cells that are primarily responsible on a daily basis for plasma iron turnover and are central in the pathogenesis of ferroportin disease (FD), the disease attributed to lack-of-function FPN1 mutations.

Aug 18, 2011 · We found that expression levels of TNF-α and IL-6 were significantly enhanced in Fpn1LysM/LysM macrophages lacking Fpn1. These studies demonstrate that Fpn1 plays important roles in macrophage iron release in vivo and in modulating innate immune responses.

Fpn1 mRNA and Fpn1 reporter constructs, expressed in cultured cells or bone marrow macrophages, responded to iron salts suggesting transcriptional activation rather than mRNA stabilization [42].

Dec 1, 2003 · To gain insight into FPN1's role in macrophage iron metabolism, we examined the effect of iron status and erythrophagocytosis on FPN1 expression in J774 macrophages. Northern analysis indicated that FPN1 mRNA levels decreased with …

Ferroportin 1 (FPN1) is an iron export protein found in mammals. FPN1 is important for the export of iron across the basolateral membrane of absorptive enterocytes and across the plasma membrane of macrophages. The expression of FPN1 is regulated by hepcidin, which binds to FPN1 and then induces its degradation.

Oct 10, 2024 · Heterozygous mutations in SLC40A1, encoding a multi-pass membrane protein of the major facilitator superfamily known as ferroportin 1 (FPN1), are responsible for two distinct hereditary iron-overload diseases: ferroportin disease, which is associated with reduced FPN1 activity (i.e., decrease in cellular iron export), and SLC40A1 -related ...