FITC-dextran is used extensively in microcirculation and cell permeability research utilizing microfluorimetry. 22,23 FITC-dextran has been used to study plant cell wall porosity 24 and capillary permeability. 25,26 Plasma proteins have been shown not to bind to FITC-dextran. 26

FITC-Dextran, also known as fluorescein isothiocyanate-labeled dextran, is a versatile molecular marker extensively applied in cell and tissue research, encompassing biochemical investigations. This compound is the result of linking the fluorescent FITC dye with dextran, a polysaccharide composed of branching glucose molecules of varied lengths. FITC-Dextrans are instrumental …

FITC-dextran is used in cardiovascular, microcirculation, perfusion, cell monolayer and cell membrane permeability research as fluorescent flux tracer compound that supports the measurement of processes such as blood flow, membrane damage, vascular drainage and …

The peripheral perfusion of a large fluorescently labeled molecule that does not normally penetrate the BBB, such as fluorescein isothiocyanate (FITC) labeled dextran, and its measurement in the brain would be a sensitive indicator of a possible disruption of the BBB.

Fluorescein isothiocyanate (FITC)-dextrans are synthesised by coupling fluoresceinyl isothiocyanate (5-isomer) to selected dextran fractions. All the batches are checked for molecular weight, degree of substitution, loss on drying and free FITC. TdB Labs produces FITC-dextrans from 4 kDa to 2000 kDa that are supplied as a yellow to orange powder.

Apr 10, 2017 · A detailed protocol is presented for assessing the integrity of the BBB by transcardial perfusion of a 10,000 Da FITC-labeled dextran molecule and its visualization to determine the degree of extravasation from brain microvessels. © 2017 by John Wiley & …

Coupling fluorescein-isothiocyanate to dextrans (FITC-D) extends the usefulness of the dextrans as electron microscopic tracer particles by permitting preceding fluorescence stereo microscopy and high-power light microscopy of the tissue specimens.

FITC-dextrans represent a valuable tool to characterize altered blood-brain barrier permeability in animal models. Diffusion and washout of low molecular weight FITC-dextran can be avoided by direct immobilization through immediate freezing of the tissue.

Jan 5, 2023 · To investigate the influence of the dose in the FITC-Dextran 4kDa (FD-4) permeability test in an obese mouse model, we tested the bodyweight dose regimen and a lean body mass-based dose...

Apr 4, 2017 · FITC-Dextran 70 is dextran labeled with fluorescein isothiocyanate for possible use in perfusion studies in animals. FITC-Dextran 70 is used as a fluorescent probe to study cell …