p44/42 MAPK (Erk1/2) Antibody #9102 - Cell Signaling …
Western blot analysis of extracts from HeLa cells transfected with 100 nM control siRNA #6201 (-) or p44 MAPK (Erk1) siRNA (+), using p44/42 MAPK (Erk1/2) Antibody #9102 and GCK Antibody #3782. The Erk1/2 antibody confirms silencing of Erk1 expression, and GCK Antibody is used to control for loading and specificity of p44 MAPK (Erk1) siRNA.
想做Erk1/2的信号通路WB，总erk和p-erk有什么区别_百度知道
想做Erk1/2的信号通路WB，总erk和p-erk有什么区别一、主体不同1、erk：指细胞外调节蛋白激酶，包括ERK1和ERK2，是将信号从表面受体传导至细胞核的关键。 2、p-erk：是信号从细胞表面转导到细胞核内部的重要传递者.
【精品解读】这是我见过的WB结果图最多的文章 - 知乎
这篇发表于 Nat Commun （ IF:11.8780）杂志上的文章 将WB实验从头贯彻到尾， 研究 黑色素瘤 细胞中 ITCH / BRAF / MEK / ERK 通路。 本文结论： 在黑色素瘤细胞中，受到细胞因子的刺激， BRAF主要通过K27位点被多聚泛素化，且不导致最终的蛋白降解。 这种非典型的泛素化作用是由ITCH E3连接酶响应c-Jun-N-末端激酶（JNK）介导的磷酸化作用而催化的。 K27位点的BRAF泛素化消除了14–3–3介导的BRAF激酶活性抑制，导致BRAF / MEK / ERK信号得以维持，进而有 …
ERK1/2 antibody (11257-1-AP) | Proteintech - ptglab
11257-1-AP targets ERK1/2 in WB, IHC, IF, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
- Reviews: 2
Anti-ERK1 + ERK2抗体(ab17942) - Abcam中文官网
Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
- Reviews: 25
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody detects endogenous levels of p44 and p42 MAP Kinase (Erk1 and Erk2) when phosphorylated either individually or dually at Thr202 and Tyr204 of Erk1 (Thr185 and Tyr187 of Erk2).
ERK 1/2 Antibody (MK1) | SCBT - Santa Cruz Biotechnology
Feb 2, 2021 · ERK 1/2 Antibody (MK1) is a mouse monoclonal IgG1 kappa light chain antibody raised against amino acids 325-345 of ERK1 of rat origin, specifically detecting both ERK1 (p44 MAPK) and ERK2 (p42 MAPK) proteins in mouse, rat, canine, and human samples by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF).
Phospho-ERK1/2 (Thr202/Tyr204) antibody (28733-1-AP)
28733-1-AP targets Phospho-ERK1/2 (Thr202/Tyr204) in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples. PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
- Reviews: 3
Erk1/2的信号通路WB，总erk和p-erk有什么区别呢? - 盖德问答
做磷酸化蛋白WB时，制样 (提蛋白)时需要加磷酸酶抑制剂和蛋白酶抑制剂。 1.之所以在做了p-Erk后做总Erk是为了说明p-Erk变化的特异性。 2.凋亡上走的话，不应该只是看MAPK的吧？ 还是先看看转录因子先。 3.做p-Erk类的检测，最好是加磷酸酶抑制剂。 罗氏有个什么 cooktail的卖。 个人认为p-Erk和Erk的区别就是活化与非活化的区别。 但有的位点是部分活化，有的时候全部活化。 那个磷酸酶抑制剂其实不加影响也不是很大。 以前做过磷酸化的东西，没加也跑得出来。 …
p-ERK抗体 (E-4) | SCBT - Santa Cruz Biotechnology
圣克鲁斯生物技术单克隆抗体可用于流式细胞术、免疫沉淀、Western blot和免疫染色等分析。 抗p-ERK抗体 (E-4)推荐用于以下应用：WB (Western blotting), IP (Immunoprecipitation), IF (Immunofluorescence), IHC (P) (Immunohistochemistry - Paraffin Embedded), ELISA (Enzyme-Linked Immunosorbent Assay). 组织提取物，核提取物和全细胞裂解物与圣克鲁斯生物技术单克隆抗体使用，作为western blotting或免疫沉淀阳性对照。 核提取物是理想的western blot分析或 …