DNase I is a nuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5'-phosphate-terminated polynucleotides with a free hydroxyl group on position 3', on average producing tetranucleotides.

Thermo Scientific DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyzes phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5'-phosphate and 3'-OH groups. The enzyme activity is strictly dependent on Ca2+ and is activated by Mg2+ or Mn2+ ions.

DNase I, (RNase-free) is an endonuclease that nonspecifically cleaves DNA to release di-, tri- and oligonucleotide products with 5´-phosphorylated and 3´-hydroxylated ends (1,2). DNase I acts on single- and double-stranded DNA, chromatin and RNA:DNA hybrids.

DNase I is a versatile enzyme that nonspecifically cleaves DNA to release 5'-phosphorylated di-, tri-, and oligonucleotide products (1). A powerful research tool for DNA manipulations, DNase I is used in a range of molecular biology applications.

DNase I is suitable for removing DNA from protein preparations, nick translating DNA, and generating random fragments for dideoxy sequencing. NOTE: for removing DNA from RNA preparations, use Amplification Grade DNase I (Cat. No. 18068-015).

Deoxyribonuclease (DNase, for short) refers to a group of glycoprotein endonucleases which are enzymes that catalyze the hydrolytic cleavage of phosphodiester linkages in the DNA backbone, thus degrading DNA.

Jun 1, 2001 · In this review, available structural data of deoxyribonucleases I (DNases I) from several mammalian species, hen, snake and frog are summarized. Comparative studies on enzymatic and immunological properties and glycosylation are discussed, and several evolutionary conclusions are presented.

DNase I, recombinant, Grade I, is a DNA-specific endonuclease that hydrolyzes phosphodiester linkages of double- and single-stranded DNA to a mixture of mono- and oligonucleotides. DNase I, recombinant, Grade I, is manufactured using state-of-the-art processes yielding animal component-free material.

Deoxyribonuclease I (DNase I) is DNA-specific endonuclease that cleaves both single-stranded DNA,double-stranded DNA and DNA-RNA hybrids, yielding 5'-phosphate-terminated polynucleotides with a free hydroxyl group on position 3' producing tetranucleotides.

Mar 1, 2021 · Amplification Grade DNase I has been purified to remove RNase activity, and is suitable for eliminating DNA from RNA preparations prior to sensitive applications, such as RT-PCR (Reverse Transcriptase - Polymerase Chain Reaction). DNase I digests double- and single-stranded DNA into oligo- and mononucleotides.