25-hydroxycholesterol 7-alpha-hydroxylase also known as oxysterol and steroid 7-alpha-hydroxylase is an enzyme that in humans is encoded by the CYP7B1 gene. [5] [6] [7] This gene encodes a member of the cytochrome P450 superfamily of enzymes.The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and …

Dec 24, 2024 · CYP7B1 (Cytochrome P450 Family 7 Subfamily B Member 1) is a Protein Coding gene. Diseases associated with CYP7B1 include Spastic Paraplegia 5A, Autosomal Recessive and Bile Acid Synthesis Defect, Congenital, 3.Among its related pathways are Bile acid and bile salt metabolism and Oxidation by cytochrome P450.Gene Ontology (GO) annotations related …

The regulation of CYP7B1, possibly a rate-determining enzyme in the alternative pathway, has not been thoroughly studied. The aims of this study were to (1) study the regulation of liver CYP7B1 by bile acids, cholesterol, adenosine 3', 5'-cyclic monophosphate (cAMP), and phorbol myristate acetate (PMA) in primary rat hepatocytes and (2 ...

CYP7B1 and reactions catalyzed. A, schematic of the 506-amino acid enzyme showing regions encoded by the six exons of the gene and the positions marked by arrows at which five introns interrupt individual codons. The heme cofactor is covalently bound to Cys 449. B, 7α-hydroxylation of substrates (1, pregnenolone; 2, dehydroepiandrosterone; 3, 25 …

The CYP7B1 gene is a member of the cytochrome P450 gene family. Enzymes produced from cytochrome P450 genes are involved in the formation and breakdown of various molecules and chemicals within cells. The CYP7B1 gene provides instructions for making an enzyme called oxysterol 7-alpha-hydroxylase. This enzyme is produced primarily in the liver and the brain.

AKR1D1 and CYP7B1 mutations in patients with inborn errors of bile acid metabolism: Possibly underdiagnosed diseases. This study provides evidence for CYP7B1 as a key regulator of three vital intracellular regulatory oxysterol levels. SPG11 and CYP7B1 were the most common cause of autosomal recessive hereditary spastic paraplegia in Greece.

CH25H–CYP7B1–RORα axis of cholesterol metabolism in chondrocytes is a crucial catabolic regulator of the pathogenesis of osteoarthritis. Osteoarthritic chondrocytes had increased levels of cholesterol because of enhanced uptake, upregulation of cholesterol hydroxylases (CH25H and CYP7B1) and increased production of oxysterol metabolites.

Feb 11, 2025 · CYP7B1-mediated 25-hydroxycholesterol degradation maintains quiescence-activation balance and improves therapeutic potential of mesenchymal stem cells. Different effects of CYP27A1 and CYP7B1 on cognitive function: Two mouse models in comparison. Oxysterol 7-alpha Hydroxylase (CYP7B1) Attenuates Metabolic-Associated Fatty Liver Disease in Mice ...

3.3. CYP7B1 Deficiency Alters Hepatic Sterol Balance in MAFLD. CYP7B1 is a critical enzyme in the alternative bile acid synthesis pathway and affects the biosynthesis of several hydroxycholesterols. Therefore, we investigated how its deficiency would affect hepatic expression of genes related to the metabolism of bile acids and hydroxycholesterols.

The CYP7B1 cytochrome P450 enzyme hydroxylates carbons 6 and 7 of the B ring of oxysterols and steroids. Hydroxylation reduces the biological activity of these substrates and facilitates their conversion to end products that are readily excreted from the body. CYP7B1 is expressed in the liver, reproductive tract, and brain and performs different physiological functions in each tissue.