活体成像中荧光染料的选择与成像 - 知乎 - 知乎专栏
Cy5.5（Ex/Em：678/701 nm）和Cy7（Ex/Em：749/776 nm）是对 分子标记 的最优选择之一；DiD（Ex/Em：644/663 nm）、DiR（Ex/Em：748/780）染料则常用于 活体成像实验 中对细胞进行标记。
In vivo tracking of cubosomes by Cy5 fluorescence. (A) IVIS …
(A) IVIS images of whole organs, namely lung, heart, brain, tumor, liver, spleen, and kidney as numbered from 1 to 7 in the image, showing uptake of Cy5 in various organs in the Affimer...
活体成像怎么定量 in vivo imaging？ - 知乎
活体动物 光学成像技术采取绝对光子数的计算方法，记录单位时间内、单位面积接受到的 光子数。 这样，不同时间、不同仪器的测量结果可以进行比较，具有绝对的定量意义。 每一个新标记的细胞株都要进行全面的定量分析才能用于 定量实验，其中包括在体外和体内固定位置细胞发光的标准曲线。 活体动物 光学成像 提供的每一种荧光酶标记的 细胞株 都做过精确全面的分析。 具体的更详细的可以加QQ1055873363，进行咨询。 那里的数据更全面。
CY5 (Sulfo-Cyanine5) | 反应染料 | MCE
Localization of the Cy5-Cb in the mice is studied by using the Cy5 fluorescence filters in the IVIS Spectrum for a duration of up to 72 h postinjection. At each time point, three mice are euthanized, and the brain, liver, kidney, spleen, heart, and lungs along with the tumor are scanned under the IVIS to quantify the Cy5 fluorescence.
In vivo tracking of bioorthogonally labeled T-cells for predicting ...
2021年1月10日 · OVA-specific cytotoxic T-cells were bioorthogonally labeled with DBCO-Cy5.5 via bioorthogonal click chemistry. Cy5.5-CTLs presented the strong NIRF signals in vitro without significant changes in the functional activity. Therapeutic responses of Cy5.5-CTLs were correlated with stronger and persisted NIRF signals from Cy5.5-CTLs at the tumor.
The dynamic process of mRNA delivery by lipid nanoparticles in vivo
2024年8月1日 · Bioluminescence imaging of luciferase and fluorescence imaging of DiR and Cy5 by IVIS Spectrum could provide real-time information on mRNA translation, LNP location, and mRNA distribution after intramuscular or intravenous injection of DiR-labeled SM-102 or MC3 LNPs-encapsulated Cy5-Luc mRNA.
Near-infrared fluorescent deoxyglucose analogue for tumor …
Tumor targeting and retention of Cy5.5-2DG and Cy5.5-NHS in a subcutaneous U87MG glioma and A375M melanoma tumor model were evaluated and quantified by a Xenogen IVIS 200 optical cooled charged-coupled device system.
Evaluation of a novel monoclonal antibody mAb109 by immuno …
2019年9月18日 · The receptor specificity of Cy5.5-mAb109 was verified by mAb109 blocking and Cy5.5-IgG (negative control) fluorescence experiments. Unlabeled mAb109 significantly reduced tumor uptake and tumor...
Applications of IVIS (In Vivo Imaging Systems) - AZoLifeSciences
2022年9月5日 · Some popular small fluorescent dyes include Cy5, Cy7, and indocyanine green. Other dyes such as SiR-NIRs have even been modified through rhodamine scaffolds, allowing for emissions that lay within a suitable range for in vivo imaging.
Biodistribution of Cy5-MGdNPs in mice imaged using a
We encapsulated gadolinium oxide (Gd2O3) nanoparticles within phospholipid micelles as a novel low cytotoxic T 1-weighted MRI imaging contrast agent (MGdNPs) that can also deliver small molecules...