This review describes three elements of cell sheet tissue engineering in terms of the chemical and physical effects of material surfaces and the interfacial properties of cell sheets: preparation, harvesting/manipulation and transplantation of cell sheets.

2004年5月1日 · We have developed ‘cell sheet engineering’ in order to avoid the limitations of tissue reconstruction using biodegradable scaffolds or single cell suspension injection. Our concept is tissue reconstruction, not from single cells, but from cell sheets. Cell sheets are prepared using temperature-responsive culture dishes.

A strategy of ‘cell sheet engineering’, in which cultured cells are harvested as intact sheets along with their deposited extracellular matrix (ECM), was developed using temperature-responsive surfaces and can be applied in tissue engineering.

Cell sheet engineering is a scaffold-free tissue engineering technique that enables the formation of cell-dense tissues without the use of enzymes [8]. The assembly of cells into sheet-like structures facilitates the delivery of a large number of cells to targeted areas.

2023年2月24日 · Cell sheet engineering is an advanced scaffold-free tissue engineering technique applicable to repairing or regenerating defective tissues and organs, including the heart , skin , cornea , cartilage , esophagus , and brain . It …

Cell sheet engineering is an advanced scaffold-free tissue engineering technique applicable to repairing or regenerating defective tissues and organs, including the heart [2, 4], skin [5], cornea [6], cartilage [7], esophagus [8], and brain [9]. It was first developed and published in 1990 by Yamada et al. [10].

2019年1月1日 · Compared with traditional cell therapies with evident shortcomings (e.g., cell suspension injection or tissue engineering with scaffolds), scaffold-free cell sheet technology enables transplanted cells to be grafted and fully maintain their viability on target sites.

2023年4月27日 · Cell sheet engineering (CSE) is a novel technique that supports enzyme-free cell detachment in the shape of a sheet-like structure. Compared with the traditional method of enzymatic digestion, products harvested by this technique retain extracellular matrix (ECM) secreted by cells as well as cell-matrix and intercellular junctions established ...

Cell sheet engineering therefore allows for tissue regeneration by either direct transplantation of cell sheets to host tissues or the creation of three-dimensional structures via the layering of individual cell sheets.

2022年11月25日 · Cell sheet engineering (CSE) has emerged as a promising strategy for scaffold-free cell-based regenerative medicine therapy by means of transplantable single or multilayered cell-dense sheets to achieve tissue repair and regeneration.