ctcf的主要功能是调控染色质的3d结构 [4] 。 ctcf和dna双链结合形成染色质环，并把dna锚定在细胞结构上（例如核纤层） [6] 。 此外，ctcf还能充当常染色质和异染色质的边界。

CTCF physically binds to itself to form homodimers, [15] which causes the bound DNA to form loops. [16] CTCF also occurs frequently at the boundaries of sections of DNA bound to the nuclear lamina. [10] Using chromatin immuno-precipitation (ChIP) followed by ChIP-seq, it was found that CTCF localizes with cohesin genome-wide and affects gene regulatory mechanisms …

ctcf是广泛存在于真核生物中的多功能转录因子.利用细胞瞬时表达实验在hela细胞系中研究ctcf各结构域的转录活性.方法:借用checkmate哺乳动物双杂交系统的2个质粒pbind和pg5luc,将ctcf的n、m、c段及全长编码序列克隆至pbind质粒的gala dna结合结构域编码序列后的 多克隆位点,将表达gala dna结合结构域-ctcf 融合 ...

2015年6月5日 · CCCTC-binding factor (CTCF) is an 82-kDa protein with 11 zinc fingers. 1 It was first identified as a transcriptional repressor of the chicken c-myc gene, a regulatory gene that encodes the c-myc ...

2014年3月11日 · CCCTC-binding factor (CTCF) is a DNA-binding protein that has various, often seemingly contradictory, roles in gene regulation. This Review describes these disparate functions and how the context ...

2025年2月28日 · CTCF is considered as the most essential transcription factor regulating chromatin architecture and gene expression. However, genome-wide impact of CTCF on erythropoiesis has not been extensively investigated. Using a state-of-the-art human erythroid progenitor cell model (HUDEP-2 and HEL cell lines), we systematically investigate the effects …

A versatile role for CTCF in chromatin biology. (a) Functional categories of CTCF binding sites across the genome, adopted from Chen et al.[](b) (i) CTCF binding sites are found at boundaries that separate active and inactive domains.CTCF binding to (ii) enhancer-like sequences and (iv) gene promoters can facilitate looping between these sequences.

The participation of CTCF and CTCF-short (CTCF-s) in the formation of chromatin loops. (a) Representation of the domain distribution of CTCF and CTCF-short (CTCF-s).(b) CTCF physically binds to itself to form homodimers which promote chromatin loop formation through cohesin ring protein.CTCF-s competes with CTCF to alter the chromatin architecture and loop formation, …

CCCTC‐binding factor (CTCF) is an eleven zinc finger (ZF), multivalent transcriptional regulator, that recognizes numerous motifs thanks to the deployment of distinct combinations of its ZFs. The great majority of the ~50,000 genomic locations bound ...

2013年5月6日 · CTCF has it all. The transcription factor binds to tens of thousands of genomic sites, some tissue-specific, others ultra-conserved. It can act as a transcriptional activator, repressor and insulator, and it can pause transcription. CTCF binds at chromatin domain boundaries, at enhancers and gene pr …