Bub3 is a protein involved with the regulation of the Spindle Assembly Checkpoint (SAC); though BUB3 is non-essential in yeast, it is essential in higher eukaryotes. As one of the checkpoint proteins, Bub3 delays the irreversible onset of anaphase through direction of kinetochore localization during prometaphase [ 5 ] to achieve biorientation.

2024年12月24日 · BUB3 (BUB3 Mitotic Checkpoint Protein) is a Protein Coding gene. Diseases associated with BUB3 include Mosaic Variegated Aneuploidy Syndrome and Seckel Syndrome . Among its related pathways are CDK-mediated phosphorylation and removal of Cdc6 and EML4 and NUDC in mitotic spindle formation .

2022年5月18日 · Upregulation of the BUB3 gene has been found in a variety of human cancers and is associated with poor prognoses. Here, we review the structure and functions of BUB3 in mitosis, its expression in cancer and association with survival prognoses, and its potential as an anticancer target.

Findings reveal a regulatory role of Bub3 in DMAP1-mediated DNA methylation upon mitotic stress. The roles for the BUB3-BUB1 complex in S phase. Small sequence differences in Bub1 and BubR1 direct Bub3 to different phosphorylated targets in the spindle assembly checkpoint signaling cascade.

2018年7月2日 · Our findings have uncovered a novel function of the plant BUB3 in MAP65-3-dependent microtubule reorganization during cytokinesis. Budding uninhibited by benzimidazole 3 (BUB3) is a WD40...

2016年5月5日 · Bub3 is a “donut-shaped” WD40 protein with a seven-blade β-propeller structure forming a symmetric circular wall around a central pore or funnel region ( 8, 9 ). It binds to phosphorylated MELT repeats (Met-Glu-Leu-Thr (P)) on the outer kinetochore subunit Knl1, acting as a scaffolding protein for its binding partners BubR1 and Bub1 ( 10, – 15 ).

2009年11月2日 · We propose that as a member of SAC, Bub3 is required for regulation of both meiosis I and II, and is potentially involved in kinetochore-microtubule attachment in mammalian oocytes.

2014年2月10日 · BuGZ binds to and stabilizes Bub3 during interphase and mitosis through a highly conserved GLE2p-binding sequence (GLEBS) domain.

2013年9月24日 · In this study, we report that Bub3, a 7-bladed β-propeller, is the MELT P reader. It contains an exceptionally well-conserved interface that docks the MELT P sequence on the side of the β-propeller in a previously unknown binding mode. Mutations targeting the Bub3 interface prevent kinetochore recruitment of the SAC kinase Bub1.

2008年1月16日 · Using RNA interference and high-resolution microscopy, we analyzed K-MT interactions in Bub3-depleted cells and compared them to those in Bub1- or BubR1-depleted cells. We found that Bub3 is essential for the establishment of correct K-MT attachments.