Human AP endonuclease (APE1), like most AP endonucleases, is of class II and requires an Mg 2+ in its active site in order to carry out its role in base excision repair. The yeast homolog of this enzyme is APN1.

ape 1 切割 ap 位点 5´ 端的磷酸二酯键，产生单链 dna 断裂，留下 3´ 羟基和 5´ 脱氧核糖磷酸末端。

In this paper we review the research status of APE1 mediated functional nucleic acids biosensing techniques and other biosensing techniques for the detection of APE1, discuss the significances and problems of APE1-relevant biosensing techniques, and prospect development trends of APE1 mediated detecting techniques for the detection of more ...

Sep 14, 2022 · Nature Communications - AP endonuclease 1 (APE1) processes genomic AP sites during base excision repair. Here, the authors determine the structural mechanism used by APE1 to...

APE1/Ref-1 (APE1), the mammalian ortholog of Escherichia coli Xth, and a multifunctional protein possessing both DNA repair and transcriptional regulatory activities, has a pleiotropic role in controlling cellular response to oxidative stress.

Jan 27, 2021 · The exonuclease activity of Apurinic/apyrimidinic endonuclease 1 (APE1) is responsible for processing matched/mismatched terminus in various DNA repair pathways and for removing...

Jan 1, 2021 · Mammalian apurinic/apyrimidinic (AP) endonuclease 1 (APE1) has versatile enzymatic functions, including redox, endonuclease, and exonuclease activities. APE1 is thus broadly associated with pathways in DNA repair, cancer cell growth, and drug resistance.

Apurinic endonuclease 1 (APE1) is one of the key enzyme in the DNA base excision repair system. APE1 hydrolyzes DNA adjacent to the 5'-end of an apurinic/apyrimidinic (AP) site to produce a nick with a 3'-hydroxyl group and a 5'-deoxyribose phosphate moiety. 无嘌呤核酸内切酶 1 (APE1) 是 DNA 碱基切除修复系统中的关键酶之一。

摘要： 脱嘌呤/脱嘧啶核酸内切酶1（Apurinic/apyrimidinic endonuclease 1,APE1）是一种广泛存在于生物体内、在碱基切除修复（Base excision repair,BER）过程中能够在碱基缺失位点（AP site）处识别并切割DNA的蛋白酶,其作用效率高且特异性强。同时,APE1在一些癌症细胞中的活性 ...

Oct 12, 2015 · We report multiple high-resolution human APE1–DNA structures that divulge new features of the APE1 reaction, including the metal-binding site, the nucleophile and the arginine clamps that ...