Quantification of epidermal growth factor receptor expression …
2015年10月6日 · In addition, the averaged A431 cell surface EGFR density was found to be 636/μm(2) with an estimation of 5 × 10(5) EGFR per cell. Additional measurement also …
A431 cells - Wikipedia
A431 cells are used in studies of the cell cycle and cancer-associated cell signalling pathways since they express abnormally high levels of the Epidermal growth factor receptor (EGFR). …
A multivalent biparatopic EGFR-targeting nanobody drug …
2021年9月3日 · Therefore, biparatopic nanobodies and its ADC, equipped with two different papratopes and two modes of EGFR inhibition, could exert potent dose-dependent antitumor …
The EGFR and HER2 expression of A431, U343, SKOV3 and
The tyrosine kinase inhibitor GEF has been reported to induce dimer formation by enhancing the affinity of EGF-EGFR, while lapatinib stabilizes the inactive form of EGFR and reduces the...
Growth stimulation of A431 cells by epidermal growth factor ...
Epidermal growth factor (EGF) at 3 nM maximally inhibits the proliferation of A431 epidermoid carcinoma cells. We show that at lower concentrations, in the range of 3-100 pM, EGF has a …
Targeting EGFR-overexpressed A431 cells with EGF-labeled
2013年4月26日 · The binding efficiency of the EGF to the nanoparticles was measured by flow cytometry using a specific anti-EGF antibody. The ability of EGF bioconjugates to target the …
Proximity extracellular protein-protein interaction analysis of EGFR ...
2023年12月14日 · To validate the performance of EGFR-FabID, we compared five cell lines with different EGFR expression levels: A431 (high), Expi293F-EGFR stable (middle), HeLaS3 …
Stimulation or inhibition of A431 cell growth by EGF is directly ...
In the presence of tyrosine kinase inhibitor coumaric acid (0.2 approximately 2 microM), A431 cell growth was stimulated, rather than inhibited, by EGF in a dose-dependent manner. In contrast, …
Acquired resistance to EGFR tyrosine kinase inhibitor in A431 …
2014年12月16日 · In the current study, we reported the tumor xenografts of the human A431 squamous cell carcinoma, after 25-week consecutive therapy with EGFR inhibitor (gefitinib) …
Consumption of EGF by A431 cells: evidence for receptor recycling
2001年1月1日 · We examined the extent of EGF consumption by EGFR in A431 cells. When 125I-EGF was added to A431 cell cultures at low or high density, at concentrations which …