FITC，英文全称 Fluorescein Isothiocyanate，中文名称异硫氰酸荧光素，是生物学中应用最为广泛的一种绿色荧光素衍生物。 FITC 具有高吸收率和优良的荧光量子产率等特点。

FITC，英文全称Fluorescein Isothiocyanate，中文名称异硫氰酸荧光素，具有高吸收率、优良的荧光量子产率和良好的水溶性等特点，是生物学中应用最为广泛的一种绿色荧光素衍生物，其异硫氰酸基团可与蛋白的氨基末端或者伯胺反应从而实现包括抗体，凝集素在内的 ...

Apr 1, 2011 · 它通常以异构体的混合物形式提供，即 5-异硫氰酸荧光素 (5-FITC) 和 6-异硫氰酸荧光素 (6-FITC)。 FITC 对亲核试剂（包括蛋白质上的胺和巯基）具有反应性。

FITC分子量为389.4，最大吸收光波长为490~495nm，最大发射光波长为520~530nm，呈现明亮的黄绿色荧光。 FITC在冷暗干燥处可保存多年，是目前应用最广泛的荧光素。

It is typically available as a mixture of isomers, fluorescein 5-isothiocyanate (5-FITC) and fluorescein 6-isothiocyanate (6-FITC). FITC is reactive towards nucleophiles including amine and sulfhydryl groups on proteins.

Mar 4, 2025 · FITC (Fluorescein 5-isothiocyanate) 是一种用于胺标记的荧光探针。 FITC 是对 pH 和 Cu2+ 敏感的荧光染料。 Annexin V-FITC/PI is employed to detect chondrocyte apoptosis. The PEI-modified CDs are conjugated with fluorescein isothiocyanate (FITC) to fabricate CDs-FITC composites. 蛋白质荧光标记试剂。 用于荧光抗体技术快速鉴定病原体。 染料和代谢物。 生化研究。 荧光抗体示踪。 病毒和寄生虫等所致疾病的快速诊断。

Apr 18, 2009 · Fluorescein 5-isothiocyanate is the 5-isomer of fluorescein isothiocyanate. Acts as a fluorescent probe capable of being conjugated to tissue and proteins; used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.

Mar 20, 2024 · 异硫氰酸荧光素 （英语： Fluorescein isothiocyanate，缩写 FITC），是一种 荧光素 衍生物类 荧光 染料，广泛应用于 流式细胞术 和 荧光显微镜，激发峰在495nm，发射峰在519nm [1]。

5-Fluorescein isothiocyanate (FITC) is yellow-orange in color with an absorption maximum at 492nm. Upon excitation, it emits a yellow-green color with an emission maximum at 520nm. It is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins.

In immuno histology/ cytology, Fluorescein-5-isothiocyanate (FITC) for biochemistry and for fluorescence microscopy, serve as marker substance for the visualization and for the detection of tissue-bound bio molecules like e.g. antigens, lectins, various proteins, peptides, nucleic acids, oligo- and polysaccharides in samples of human origin.