Standard E. coli vector with a multiple cloning site (MCS) for DNA cloning. The MCS is reversed in pUC19. Explore Over 2.7k Plasmids: Basic Cloning Vectors | More Plasmid Sets. Sticky ends from different EcoO109I sites may not be compatible. Sticky ends from different TsoI sites may not be compatible.

Plasmid pUC18 from Dr. Joachim Messing's lab is published in Gene. 1983 Dec;26(1):101-6. This plasmid is available through Addgene.

pUC18 is a commonly used plasmid cloning vector in E.coli. The molecule is a double-stranded circular DNA (2686 base pairs in length). Due to a small size pUC18 enables successful cloning of large DNA fragments. The pUC18 plasmid confers ampicillin resistance and complement defects in β-galactosidase in appropriate host strains. The

For instance, the pUC18 vector expresses the lacZα gene encoding the alpha peptide of beta-galactosidase which, in combination with X-gal, allows color selection of bacterial colonies formed after cloning (learn more about blue/white selection in colony screening).

Plasmid pUC18T-mini-Tn7T-Gm from Dr. Herbert Schweizer's lab is published in Nat Methods. 2005 Jun;2 (6):443-8. This plasmid is available through Addgene.

2020年1月16日 · pUC18 is one of the most widely used cloning and expression vectors within research labs today. The pUC series (containing pUC18, 19) was created at the University of California by Yanisch-Perron et al . as a modification of an existing group of cloning plasmids .

Thermo Scientific pUC18 vector is a small, high copy number, E. coli plasmid, 2686 bp in length. It contains identical multiple cloning site (MCS) as pUC19 vector except that it is arranged in opposite orientation. Highlights • Purified by chromatography …

The pUC18 and pUC19 plasmids enable successful cloning of large DNA fragments (larger than those cloned with a M13 mp18 RF Phage Vector).

Plasmid pUC18-GFP from Dr. Sébastien Britton's lab contains the insert EGFP and is published in bioRxiv [Preprint]. 2024 Jan 4:2024.01.03.574002. doi: 10.1101/2024.01.03.574002. This plasmid is available through Addgene.

puc18/19 vector map multiple cloning sites of puc18: m13 hindlll bevl 5’ g taa aac gac ggc cag tgc caa gct tgc atg cct gca ggt cga ctc tag agg atc ccc ggg tac cga gct cga att c 3’ c att ttg ctg ccg gtc acg gtt cga acg tac gga cgt cca gct gag atc tcc tag ggg ccc atg gct cga gct taa g