pGEX-4T-1 Sequence and Map - SnapGene
Bacterial vector for expressing fusion proteins with a thrombin site. For other reading frames, use pGEX-4T-2 or pGEX-4T-3.
pGEX Vectors - MilliporeSigma
This page describes the characteristics of pGEX expression vectors used with Cytiva products.
The pGEX vectors are designed for inducible, high-level intracellular expression of genes or gene fragments as fusions with Schistosoma japonicum GST. Expression in E. coli yields tagged …
pGEX Vectors (GE Healthcare) - SnapGene
Discover the most user-friendly molecular biology experience.
Preparation of Competent Cells and Transformation with pGEX …
In these procedures, E. coli host cells are made competent and then transformed with either uncut pGEX DNA or recombinant pGEX DNA. If electroporation is used to transform the cells, see …
pGEX-3X Sequence and Map - SnapGene
Bacterial vector for expressing GST fusions with a Factor Xa site. Sequence Author: GE Healthcare. Explore Over 2.7k Plasmids: pGEX Vectors (GE Healthcare) | More Plasmid Sets. …
pGEX vectors are to be used for scientific investigation and research and for no other purpose whatsoever and a license for commercial use of the licensed products and the processes …
Prepare fusion construct by inserting gene of interest into the multiple cloning site of pGEX-4T-1 using any one, or combination of unique restriction sites and transform into a host of choice …
pGEX Vectors - Cytiva
PreScission Protease, Thrombin, or Factor Xa recognition sites for cleaving the desired protein from the fusion product. Thirteen pGEX vectors are available (see Figure). Nine of the vectors …
Troubleshooting in pGEX Expression Vectors - MilliporeSigma
This page describes troubleshooting strategies for cloning the gene or gene fragment into a pGEX expression vector.
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