Bacterial vector for expressing fusion proteins with a thrombin site. For other reading frames, use pGEX-4T-2 or pGEX-4T-3.

This page describes the characteristics of pGEX expression vectors used with Cytiva products.

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The pGEX vectors are designed for inducible, high-level intracellular expression of genes or gene fragments as fusions with Schistosoma japonicum GST. Expression in E. coli yields tagged proteins with

In these procedures, E. coli host cells are made competent and then transformed with either uncut pGEX DNA or recombinant pGEX DNA. If electroporation is used to transform the cells, see Appendix 3 (Electroporation). Otherwise, proceed as described below.

Bacterial vector for expressing GST fusions with a Factor Xa site. Sequence Author: GE Healthcare. Explore Over 2.7k Plasmids: pGEX Vectors (GE Healthcare) | More Plasmid Sets. Sticky ends from different BtgI sites may not be compatible. Sticky ends from different Bsu36I sites may not be compatible.

From recombineering to CRISPR, this collection contains a variety of tools to help you harness bacterial genomes for your research. Bacterial expression of Cas9 nuclease gRNA. For a more comprehensive list of our bacterial CRISPR plasmids, see here.

This page describes troubleshooting strategies for cloning the gene or gene fragment into a pGEX expression vector.

Prepare fusion construct by inserting gene of interest into the multiple cloning site of pGEX-4T-1 using any one, or combination of unique restriction sites and transform into a host of choice such as E. coli BL21 (27154201).

Collectively, the pGEX vectors provide all three translational reading frames beginning with the EcoRI restriction site (Figure 1.1). pGEX-1λT, pGEX-6P-1, pGEX-4T-1, and pGEX-5X-1 can directly accept and express cDNA inserts isolated from λgt11 libraries.