Bacterial vector for expressing fusion proteins with a thrombin site. For other reading frames, use pGEX-4T-2 or pGEX-4T-3.

This page describes the characteristics of pGEX expression vectors used with Cytiva products.

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The pGEX vectors are designed for inducible, high-level intracellular expression of genes or gene fragments as fusions with Schistosoma japonicum GST. Expression in E. coli yields tagged …

In these procedures, E. coli host cells are made competent and then transformed with either uncut pGEX DNA or recombinant pGEX DNA. If electroporation is used to transform the cells, see …

Bacterial vector for expressing GST fusions with a Factor Xa site. Sequence Author: GE Healthcare. Explore Over 2.7k Plasmids: pGEX Vectors (GE Healthcare) | More Plasmid Sets. …

From recombineering to CRISPR, this collection contains a variety of tools to help you harness bacterial genomes for your research. Bacterial expression of Cas9 nuclease gRNA. For a …

This page describes troubleshooting strategies for cloning the gene or gene fragment into a pGEX expression vector.

Prepare fusion construct by inserting gene of interest into the multiple cloning site of pGEX-4T-1 using any one, or combination of unique restriction sites and transform into a host of choice …

Collectively, the pGEX vectors provide all three translational reading frames beginning with the EcoRI restriction site (Figure 1.1). pGEX-1λT, pGEX-6P-1, pGEX-4T-1, and pGEX-5X-1 can …