
pGEX-4T-1 Sequence and Map - SnapGene
Bacterial vector for expressing fusion proteins with a thrombin site. For other reading frames, use pGEX-4T-2 or pGEX-4T-3.
pGEX Vectors - MilliporeSigma
This page describes the characteristics of pGEX expression vectors used with Cytiva products.
pGEX Vectors (GE Healthcare) - SnapGene
Discover the most user-friendly molecular biology experience.
The pGEX vectors are designed for inducible, high-level intracellular expression of genes or gene fragments as fusions with Schistosoma japonicum GST. Expression in E. coli yields tagged …
Preparation of Competent Cells and Transformation with pGEX …
In these procedures, E. coli host cells are made competent and then transformed with either uncut pGEX DNA or recombinant pGEX DNA. If electroporation is used to transform the cells, see …
pGEX-3X Sequence and Map - SnapGene
Bacterial vector for expressing GST fusions with a Factor Xa site. Sequence Author: GE Healthcare. Explore Over 2.7k Plasmids: pGEX Vectors (GE Healthcare) | More Plasmid Sets. …
Bacterial Expression Systems - Addgene
From recombineering to CRISPR, this collection contains a variety of tools to help you harness bacterial genomes for your research. Bacterial expression of Cas9 nuclease gRNA. For a …
Troubleshooting in pGEX Expression Vectors - MilliporeSigma
This page describes troubleshooting strategies for cloning the gene or gene fragment into a pGEX expression vector.
Prepare fusion construct by inserting gene of interest into the multiple cloning site of pGEX-4T-1 using any one, or combination of unique restriction sites and transform into a host of choice …
pGEX Vectors - Sigma-Aldrich
Collectively, the pGEX vectors provide all three translational reading frames beginning with the EcoRI restriction site (Figure 1.1). pGEX-1λT, pGEX-6P-1, pGEX-4T-1, and pGEX-5X-1 can …