The fundamental question in the analysis of gene expression at the RNA level is whether RNA sequences derived from a gene of interest are present in cells or tissues. Detecting specific RNA sequences can be accomplished by Northern blotting, the whimsically named analog of Southern blotting, when applied to RNA analysis.

Analytical methods for mRNA characterisation, lot release and stability testing of mRNA drug substance and drug product must be developed and performed to monitor critical quality attributes (CQAs). mRNA is a highly polar molecule due to its …

In this chapter, we review the technical aspects of the current methods of RNA-Seq, both short and long-read approaches, and data analysis methods available. We discuss recent advances in single-cell RNA-Seq and direct RNA-Seq approaches, which perhaps will dominate the future of …

2023年9月21日 · Here we describe a streamlined method to analyse mRNA vaccines and therapies using long-read nanopore sequencing. Compared to other industry-standard techniques, VAX-seq can comprehensively...

2022年5月12日 · Large RNA including mRNA (mRNA) has emerged as an important new class of therapeutics. Recently, this has been demonstrated by two highly efficacious vaccines based on mRNA sequences encoding for a modified version of the SARS-CoV-2 spike protein.

Analysis - PCA, UMAP, tSNE values, and graph-based clustering results with associated metadata (TSV). Differential gene expression - DEG information comparing cells from one cluster to the rest of the cells (TSV).

QC Metric Guidelines mRNA total RNA RNA Type(s) Coding Coding + non-coding RIN > 8 [low RIN = 3’ bias] > 8 Single-end vs Paired-end Paired-end Paired-end Recommended Sequencing Depth 10-20M PE reads 25-60M PE reads FastQC Q30 > 70% Q30 > 70% Percent Aligned to Reference > 70% > 65% Million Reads Aligned Reference > 7M PE reads (or > 14M reads) > …

2022年2月2日 · Analytical approaches must keep pace to ensure the identity, safety, and efficacy of evolving mRNA candidates. The success of the messenger RNA (mRNA) vaccines against the SARS-CoV-2 virus brought attention to a field that has steadily advanced for many years.

2025年2月3日 · We trained mRNA-LM on millions of diverse mRNA sequences from several different species. The unsupervised model was able to learn meaningful biology related to evolution and host–pathogen interactions. Fine-tuning of mRNA-LM allowed us to use it in several mRNA property prediction tasks.

There are four main types of RNA therapeutics: . mRNA, antisense RNA (asRNA), RNA interference (RNAi) and RNA aptamers. Of the four, only mRNA-based therapeutics involves delivery of in vitro transcribed mRNA into a target cell, where cellular machinery translates the mRNA into a protein.