NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free. An E. coli strain that carries the XhoI gene from Xanthomonas holcicola (ATCC 13461).

Thermo Scientific XhoI restriction enzyme recognizes C^TCGAG sites and cuts best at 37°C in R buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes.

In molecular biology, XhoI is a type II restriction enzyme EC that recognise the double-stranded DNA sequence CTCGAG and cleaves after C-1. [1] Type II restriction endonucleases are components of prokaryotic DNA restriction-modification mechanisms that

Restriction enzyme that cuts DNA at 37°C and leaves a 5´ overhang. R6051, R6055

Thermo Scientific FastDigest XhoI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers. The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change or subsequent DNA clean-up ...

DNA digestion with XhoI may be affected by the following types of methylation: cpg (Impaired). † For convenience, 1.0 µl is specified; adjust as needed. In general, we recommend 5–10 units of enzyme per µg DNA, and 10–20 units for genomic DNA in a 1 hour digest.

Thermo Scientific Tango Buffer is provided to simplify buffer selection for double digests. 98% of Thermo Scientific restriction enzymes are active in a 1X or 2X concentration of TangoTM Buffer. Please refer to www.thermoscientific.com/doubledigest to choose the best buffer for your experiments. 0.1 mg/mL BSA.

Invitrogen™ Anza™ 8 XhoI is a restriction enzyme that cuts DNA at this recognition site: C^TCGAG, completely digesting the DNA in 15 minutes at 37°C. Isoschizomers include: PaeR7I, Sfr274I, SlaI. For superior convenience, a single buffer and protocol are used with all Anza™ restriction enzymes.

Thermo Scientific XhoI restriction enzyme recognizes C^TCGAG sites and cuts best at 37°C in R buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes.

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. Lambda DNA digested with XhoI, 0.7% agarose, 1 cleavage sites.