Wheat Germ Agglutinin (WGA) - Thermo Fisher Scientific
WGA is a cell impermeant stain that selectively binds to N‐acetylglucosamine and N‐acetylneuraminic acid (sialic acid) residues, which are often found on cell membranes. Fluorescent WGA conjugates provide selective labeling of the plasma membrane with minimal background in many cell types that is retained after formaldehyde fixation and ...
Wheat Germ Agglutinin (WGA) Conjugates - Biotium
WGA is commonly used to label glycoproteins for imaging of the plasma membrane in live or fixed cells, for staining of tissue sections, or for western blotting. WGA can be used as a gram stain to fluorescently label gram bacteria but not gram- bacteria.
Wheat germ agglutinin - Wikipedia
Wheat germ agglutinin (WGA) is a lectin that protects wheat from insects, yeast and bacteria. An agglutinin protein, it binds to N-acetyl-D-glucosamine and sialic acid. [1] WGA has also been shown to interact with sialic acid residues on oligosaccharides. [2]
Alvetex Protocol: Wheat Germ Agglutinin Staining - REPROCELL
Wheat Germ Agglutinin (WGA) is a well-known lectin originally noted for its differential ability to agglutinate cancerous versus normal cells[1] and later identified as a plasma membrane staining agent of both animal[2] and bacterial[3] cells, thanks to its binding of N-acetyl-D-glucosamine and N-acetylneuraminic acid residues[4] on plasma ...
Plasma Membrane Structure | Thermo Fisher Scientific - US
Fluorescent labeled lectins (e.g., wheat germ agglutinin (WGA)) can identify cell types or cellular components in live and fixed cells. Fluorescent fusion proteins can be used to follow cellular dynamics in live cells or for end-point analysis. Some plasma membrane stains can also be used as a segmentation tool for HCS assays.
Wheat germ agglutinin selectively binds to N‐acetylglucosamine and N‐acetylneuraminic acid (sialic acid) residues.1 In solution, wheat germ agglutinin exists as a heterodimer with a molecular weight of approximately 38,000 daltons and is normally cationic under physiological conditions.
WGA is a probe for migrasomes | Cell Discovery - Nature
2019年2月26日 · In GA-fixed cells, we found that the optimum working concentration of WGA for migrasome staining was 1 µg/ml (Supplementary Fig. S2b). Using this concentration, staining for as short as 1 min...
Wheat Germ Agglutinin - an overview | ScienceDirect Topics
Wheat germ agglutinin (WGA) is one of the best-characterized wheat proteins due to its ability to bind N-acetylglucosamine (GlcNAc), including the polymeric form, chitin (β-1-4-linked GlcNAc).
Wheat Germ Agglutinin (WGA) Tracing: A Classic Approach for
2014年10月21日 · Triple color tracing of axons in the cerebellar peduncles using WGA-Alexa 555 (red), WGA-Alexa 488 (green), and WGA-Alexa 350 (blue). The staining is very bright, axons are revealed with excellent clarity, and when used in combination, there …
Wheat Germ Agglutinin (WGA) iFluor™ Conjugates - STEMCELL
Wheat germ agglutinins (WGA) are lectins—or carbohydrate-binding proteins—that have a high affinity for N-acetyl-D-glucosamine and sialic acid present in the cell membranes. Their iFluor™ conjugates are used to stain cell membranes and fibrotic scar …