Ubiquitin modification of target lysine residues typically marks substrates for proteasome-dependent degradation. However, ubiquitylation can also alter protein function through modulation of protein complexes, localization, or activity, without impacting protein turnover.

2021年7月29日 · Post-translational modification (PTM) of proteins with the small protein ubiquitin or other ubiquitin-like proteins (UBLs) provides a mechanism for the diversification of protein structure and...

2021年1月16日 · Identification of different ubiquitin-protein conjugates such as monoubiquitylation, multi-monoubiquitylation, and different types of polyubiquitin chain linkages in a specific signaling pathway is informative for the functional study of the ubiquitylation events.

2021年1月14日 · Ubiquitin modifications control a plethora of vital cellular processes through proteolytic and nonproteolytic mechanisms, including proteasomal degradation and proteostasis, selective autophagy,...

2003年7月20日 · We report here a proteomics approach to enrich, recover, and identify ubiquitin conjugates from Saccharomyces cerevisiae lysate. Ubiquitin conjugates from a strain expressing 6xHis-tagged ...

Ubiquitin modification of target lysine residues typically marks substrates for proteasome-dependent degradation. However, ubiquitylation can also alter protein function through modulation of protein complexes, localization or activity, without impacting protein turnover.

In recent years, mass spectrometry has emerged as a powerful technology for the detection and characterization of protein ubiquitination. Herein we detail qualitative and quantitative proteomic methods using a compare/contrast approach to highlight their strengths and weaknesses.

2022年3月30日 · We review various methods for enriching and quantifying ubiquitin modifications at the peptide or protein level, outline MS acquisition, and data processing approaches and discuss key challenges. Finally, we examine how MS-based ubiquitinomics can aid both basic biology and drug discovery research.

2020年1月25日 · Ubiquitination is a post-translational modification where ubiquitin is covalently attached to lysine residues on substrate proteins to signal their degradation by the 26S proteasome or initiate other non-degradation functions such as cellular trafficking.

2018年9月22日 · Here we provide a detailed method to interrogate the ubiquitin-modified proteome from any eukaryotic organism or tissue. Protein posttranslational modifications (PTMs) impart critical regulatory control on nearly every cellular process.