RP-HPLC samples will likely contain aqueous trifluoroacetic acid and acetonitrile, since these are the most commonly used mobile phases (see Basic Protocol 5). The NH 4 HCO 3 solution should be prepared fresh, as the pH of the solution will increase with age at room temperature.

The main feature of SPEED is the use of pure trifluoroacetic acid (TFA; pKa = 0.2) to dissolve cells and tissues within a few minutes and form clear lysates. After neutralization with a weak base (Tris(hydroxymethyl)-aminomethane, pKa = 8.1), the lysates become slightly turbid due to protein precipitation as fine particles.

SPEED is a universal method for peptide generation from various sources and is easily applicable even for lysis-resistant sample types as pure trifluoroacetic acid (TFA) is used for highly efficient protein extraction by complete sample dissolution.

2020年1月1日 · The new method is termed sample preparation by easy extraction and digestion (SPEED) and consists of three mandatory steps, namely acidification, neutralization and digestion. SPEED uses neither detergents nor chaotropic agents for protein extraction but pure trifluoroacetic acid.

2020年6月1日 · Trifluoroacetic acid (TFA) is used as an ion paring agent in reversed-phase chromatography to improve peak shape and resolution. The silica used in columns may have metal ion impurities, which can cause peak tailing and loss of resolution.

SPEED is a universal method for peptide gen-eration from various sources and is easily applicable even for lysis-resistant sample types as pure trifluoroacetic acid (TFA) is used for highly efficient protein extraction by complete sample dissolution.

In this application note, we used RFIC to determine the concentration of residual trifluoroacetate (TFA) in samples of interest to the pharmaceutical and biotechnology industries. TFA is commonly used during the purification of pharmaceutical and biotechnology products.

SPEED is a one-pot method for peptide generation from various sources and is easily applicable even for lysis-resistant sample types as pure trifluoroacetic acid (TFA) is used for highly efficient protein extraction.

TFA (trifluoroacetic acid) is a commonly used mobile phase additive for reversed-phase HPLC (RP-HPLC) separations of proteins and peptides. However, TFA interferes with and significantly reduces the LC/MS signal, lowering sensitivity. The ideal column for mod-ern RP-LC/MS analysis should provide symmetrical peak shape without TFA in the mobile ...

easily applicable even for lysis-resistant sample types as pure trifluoroacetic acid (TFA) is used for highly efficient protein extraction by complete sample dissolution. The protocol is