Type II topoisomerases are topoisomerases that cut both strands of the DNA helix simultaneously in order to manage DNA tangles and supercoils. They use the hydrolysis of ATP, unlike Type I topoisomerase. In this process, these enzymes change the linking number of circular DNA by ±2.

Type II topoisomerases are able to change DNA supercoiling, as well as unlink (decatenate) or link (catenate) DNA molecules. Type II topoisomerases are found in organisms of all domains of life and are encoded in most, except for a few extremely reduced ones, sequenced genomes of cellular organisms [10, 11].

2023年2月27日 · Type II Topoisomerase Functions. It increases the disentanglement of the chromosome. It does not aid in the supercoiling of DNA but is involved in their relaxation. DNA gyrase promotes the negative supercoils of DNA. One of the most important functions is that it brings the change of two units in the linking number of loops in DNA.

2009年4月20日 · Type II topoisomerases use a two gate mechanism for carrying out topological changes in DNA. The enzyme requires ATP hydrolysis for its reaction. ATP hydrolysis is used for for conformational...

4 天之前 · Understanding topoisomerase function highlights their importance in preserving chromosomal integrity and preventing genomic instability, which can lead to diseases, including cancer. ... essential for bacterial DNA compaction and replication. Eukaryotic topoisomerase II plays a significant role in chromosome segregation during mitosis. A study ...

DNA topoisomerases are enzymes that solve these difficulties by introducing transient breaks in DNA. The transient breaks allow changes in DNA topology that eliminate the overwinding. There are two classes of topoisomerases: type I enzymes introduce single strand breaks in DNA and type II topoisomerases introduce double strand breaks 1,2.

2024年5月15日 · Topoisomerase II facilitates the relaxation of supercoiled DNA. It plays a crucial role in DNA replication , transcription, and recombination. It resolves DNA knots and tangles to maintain genomic stability.

2023年11月25日 · During cell division, topoisomerase II plays a vital role in condensation and segregation of chromosomes. They help correctly distribute the parent chromosome to the two daughter cells, preventing errors.

2024年6月2日 · Type II Topoisomerases (Topoisomerase ii): Type II topoisomerases are ATP-dependent enzymes that break and rejoin both strands of the DNA molecule. These enzymes are capable of resolving more complex topological problems, such as …

Topoisomerase 2 (TOP2) regulation of DNA topological strain is critical for transcription, DNA replication, and recombination [2 – 4]. The mammalian Type IIA topoisomerases, TOP2α and TOP2β, resolve DNA topology by creating paired DNA strand breaks in a DNA duplex, and gating passage of a second DNA duplex.