The T-RFLP technique is a culture-independent, rapid, sensitive and reproducible method of assessing diversity of complex communities without the need for any genomic sequence information. The technique provides information on a collection of microorganisms that may be present in a given sample. After the completion of a T-RFLP project, if a ...

Terminal restriction fragment length polymorphism (T-RFLP) uses PCR amplification of DNA using well defined primer pairs that have been labeled with fluorescent tags. The amplified PCR products are then digested using well defined restriction enzymes.

Terminal restriction fragment length polymorphism (T-RFLP) analysis is a method for rapid profiling of mixed populations of an homologous amplicon (i.e., diverse sequences of a single gene). It combines restriction fragment analysis of a PCR-amplified gene marker with automated sequencing gel technology.

Blackwood et al. discuss the statistical analysis of terminal restriction fragment length polymorphism (T-RFLP) data. They sampled several soils and identified methods that correctly grouped replicate samples (cluster analysis) and successfully distinguished between site differences (redundancy analysis).

Terminal restriction fragment-length polymorphism (T-RFLP) is a PCR-based genetic fingerprinting technique for the study of microbial community structure based on variation in the 16S rRNA gene. These DNA fragments are commonly separated using capillary electrophoresis.

We describe TRiFLe, a freely accessible computer program that generates theoretical terminal restriction fragments (T-RFs) from any user-supplied sequence set tailored to a particular group of organisms, sequences from clone libraries, or sequences from specific genes.

Terminal restriction fragment length polymorphism is a recent molecular approach that can assess subtle genetic differences between strains as well as provide insight into the structure and function of microbial communities.

2014年1月1日 · T-RFLP profiling is a very effective method for comparing many samples in an environmental microbiology study, because fingerprints of microbial diversity can be generated in a sensitive, reproducible, and cost-effective manner. This protocol describes the steps...

T-RFLP is a PCR based genetic fingerprinting technique for the study of microbial community structure based on variation in the 16S rRNA gene. These DNA fragments are commonly separated using capillary electrophoresis.

Terminal restriction fragment length polymorphism (T-RFLP) analysis is a popular high-throughput fingerprinting technique used to monitor changes in the structure and composition of microbial communities.