19 hours ago · The current work relates to the optimization of the microbial fermentation process through Response Surface Methodology (RSM) approach for improving the soil compressive strength by Microbially Induced Calcite Precipitation (MICP). The ureolytic bacteria selected for the study were Geobacillus stearothermophilus and Acetobacter xylinum, based on maximum …

19 hours ago · Microbial community analysis during Jiuyao fermentation. A. Microbial community structure based on metagenomics analysis during the fermentation process (maximum relative abundance > 0.1%). B. Relative abundances of microbial populations determined by culture-dependent methods during Jiuyao fermentation.

5 days ago · Co-culture submerged fermentation by lactobacillus and yeast more effectively improved the profiles and bioaccessibility of phenolics in extruded brown rice than single-culture fermentation

19 hours ago · This study developed an inoculum culture for semi-controlled coffee fermentation using lactic acid bacteria (LAB) and yeast, with coffee production by-products as carbon sources. The viability of the inoculum was optimized by using a mixture design to vary the proportions of coffee pulp (CP) and wastewater (CWW) in 0.25 increments; as a process variable, …

2 days ago · Fermentation Studies in 7, 100, and 5000 L Bioreactor. The preliminary seed culture was prepared similarly to the shake flask method. Then 1 mL of preliminary seed culture was transformed to 100 mL of fresh LB60 in a 500 mL conical flask and cultured at 37 °C, 200 rpm for nearly 10 h until an OD 600 of 3–5 was reached. The secondary culture ...

2 days ago · One effective method is the application of hot water treatment to degrade the waste structure and extract its components for submerged fermentation. This method offers various advantages, such as ...

19 hours ago · For aerobic cultures, bacteria grow in standard culture tubes. For anaerobic ones, the surface of the freshly sterilized culture tube is sealed with 3 ml sterile liquid paraffin to isolate air and create an anaerobic environment. ... (ldh, pflB, and pdh) in pyruvate metabolism were determined using the relative quantification method (ΔΔCT) as ...

19 hours ago · Pasteurization usually has a great influence on yogurt and astaxanthin. This study aimed to investigate the effects of 3 pasteurization methods, including 63°C for 30 min (LTLT-1), 65°C for 30 min (LTLT-2), and 75°C for 15 s (HTST), on the physicochemical properties, stability, and biological activity of set yogurt fortified with astaxanthin-rich yolk. The results showed that …

3 days ago · This protocol describes the use of a mixed broth culture, where the culture contains several different bacterial species or strains. The specimen streaked on a plate could come in a variety of forms, such as solid samples, liquid samples, and cotton or foam swabs.

4 days ago · The commercial growth factors (GFs) and serum proteins (SPs) contribute to the high cost associated with the serum-free media for cultivated meat production. Producing recombinant GFs and SPs in scale from microbial cell factories can reduce the cost of culture media. Escherichia coli is a frequently employed host in the expression recombinant GFs and …