Significant late apoptotic positive results were obtained when the cells were assayed for apoptosis using Annexin V / PI double staining (BD Pharmingen, Oakville, ON, Canada) by using Accuri C6...

2020年1月14日 · One of the most widely used assays to determine apoptosis by flow cytometry is the estimation of fractional DNA content (aka sub-G1 assay). During apoptosis, genomic DNA is cleaved into smaller fragments, each approximately 180 bp (and multiples of it).

In Figure 6, we have a hypothetical DNA histogram with a G1 peak and sub-G1 peak. The plates represent whole cells and are in the G1 phase. Now, the sub-G1 peak represents cells undergoing apoptosis, or being “broken”.

Apoptotic cells are recognized either on the basis of their reduced DNA-associated fluorescence as cells with diminished DNA content (sub-G1) or morphologic changes. Activation of caspases can be examined through a variety of methods: colorimetric, immunoblot, or immunohistochemical.

SubG1 gate underestimates the number of apoptotic cells as cells can dye from all cell cycle phases and hypodiploid cells can temporarily accumulate above G1 phase. DNA content histogram obtained by this method can only be used to estimate subG1 cells and cannot be used

2023年1月2日 · We found that AGA significantly inhibited the proliferative capacity and metastasis of colon cancer cells in-vitro. In addition, AGA induced cell cycle arrest in the sub-G1 phase through upregulating p21 and downregulating CDK2, CDK6 in SW620, and CDK4 in SW480 and HT29, respectively.

sub-G1 is characteristic for low DNA level e.g. apoptotic cells, apoptotic bodies... senescent cells often are charterized as polyploid cells so in my opinion you should connect them with the...

2023年8月8日 · G1 Phase (Gap 1): This is the phase right after cell division. Cells increase in size, produce RNA and synthesize proteins. Importantly, this phase ensures that everything is in place for DNA synthesis to occur in the next phase. S Phase (Synthesis): During this phase, the cell’s DNA replicates.

The cell cycle characteristics of untreated KG1a cells were quantified as follows: sub-G1 0.4%, G0 0.8%, G1 67.9%, S 14.9%, G2 14.2% and M 1.8% phase. We verified the pro-apoptotic and anti-proliferative effects of exposure to camptothecin for 6 h, which induced a decrease in S, G2 and M phases (6.1%, 6.2% and 0.4%, respectively) and an ...

Technically, G1 shifts into sub-G1 because of DNA flocculation (partial relaxation of DNA: which results in lower fluorescence from PI bound to DNA) during necrosis.