The CRISPR-Cas system of Streptococcus pyogenes: function …
2022年7月20日 · Here we review how the Cas9 nuclease mediates anti-phage immunity and how it can be repurposed for the genetic engineering of human cells and other eukaryotic …
CRISPR-Cas in Streptococcus pyogenes - PMC - PubMed Central …
We review the current literature about the CRISPR-Cas systems present in S. pyogenes (types I-C and II-A), and describe their distinctive biochemical and functional features. Finally, we …
The CRISPR-Cas system of Streptococcus pyogenes: function …
2016年4月7日 · In this chapter, we review how the Cas9 nuclease mediates anti-phage immunity and how it can be repurposed for the genetic engineering of human cells and other eukaryotic …
CRISPR-Cas in Streptococcus pyogenes - PubMed
We review the current literature about the CRISPR-Cas systems present in S. pyogenes (types I-C and II-A), and describe their distinctive biochemical and functional features. Finally, we …
A dynamic subpopulation of CRISPR–Cas overexpressers allows
2024年7月12日 · In Streptococcus pyogenes, a non-canonical guide RNA tracr-L directs Cas9 to autorepress its own promoter. Here we describe a dynamic subpopulation of cells harbouring …
CRISPR-Cas in Streptococcus pyogenes - Taylor & Francis Online
2019年3月11日 · We review the current literature about the CRISPR-Cas systems present in S. pyogenes (types I-C and II-A), and describe their distinctive biochemical and functional …
Review, analysis, and optimization of the CRISPR Streptococcus pyogenes ...
2021年3月1日 · Despite certain defects hindering its clinical application, the CRISPR (clustered regularly interspaced short palindromic repeats)-associated (Cas) Streptococcus pyogenes …
Evolution of CRISPR-associated endonucleases as inferred from
2023年1月2日 · Although Streptococcus pyogenes CRISPR–Cas9 has been widely studied and repurposed for applications including genome editing, its origin and evolution are poorly …
CRISPR-Cas9D10A nickase-based genotypic and phenotypic
2016年4月15日 · Recently, the Streptococcus pyogenes type II CRISPR-Cas9 system has been adapted to perform genome engineering by inducing DNA double-strand breaks (DSBs) that …
Transcription modulation of pathogenic streptococcal and …
2024年9月19日 · We will review recent examples of CRISPRi technology in Enterococcus spp., Streptococcus pyogenes, Streptococcus agalactiae, and Streptococcus pneumoniae. We will …