The Actin Cytoskeleton and Focal Adhesion Staining Kit consists of three components (TRITC-conjugated Phalloidin, anti-Vinculin and DAPI) for the immunofluorescent staining of actin filaments in the cytoskeleton, focal contacts as well as the nucleus of the cells. Reagents and …
Immunofluorescence Staining of Cell-Cell and Focal Adhesions
Comprehensive protocol for fixation, permeabilization, and immunostaining of E-cadherin, paxillin, and vinculin in cells to visualize cell-cell junctions and focal adhesions.
Mammalian Cell Staining - protocols.io
2019年2月18日 · This protocol details how to stain mammalian cells cultured on a 96-well plate. Actin filaments, focal adhesion sites (as indicated by the presence of vinculin), and nuclei will...
Immunofluorescent Staining Protocol for Cytoskeleton and Focal …
Detailed protocol for visualizing the cytoskeleton and focal adhesions in cells using immunofluorescent staining techniques.
Focal Adhesion Isolation Assay Using ECM-Coated Magnetic …
- 1. Vortex bead vial for approximately 45 s before resuspending and transferring the desired volume of beads to a 1.5 ml conical tube. For this description, we functionalize 1 ml (approximately 4 ×...
- 2. Place the tube on the magnetic separator for 1 min and remove the supernatant.
- 3. Remove from magnet and resuspend in 1 ml buffer 1.
- 1. Vortex bead vial for approximately 45 s before resuspending and transferring the desired volume of beads to a 1.5 ml conical tube. For this description, we functionalize 1 ml (approximately 4 ×...
- 2. Place the tube on the magnetic separator for 1 min and remove the supernatant.
- 3. Remove from magnet and resuspend in 1 ml buffer 1.
- 4. Repeat steps 2 and 3.
- 作者: Wesley Sturgess, Vinay Swaminathan
- Publish Year: 2021
Immunofluorescent Staining and Focal Adhesion Quantification
Detailed protocol for immunostaining, confocal imaging, and focal adhesion analysis in cultured cells.
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Mild fixation and permeabilization protocol for preserving …
Comparing live-cell microscopic pictures of the same cell with immunofluorescences after fixation and permeabilization revealed improved results by the use of saponin. On the other hand, we …
Step-by-step quantitative analysis of focal adhesions
2014年1月1日 · Focal adhesions (FAs) are specialized adhesive structures which serve as cellular communication units between cells and the surrounding extracellular matrix. FAs are …
Protocol Isolation and culturing of primary mouse astrocytes for …
2021年12月17日 · In this protocol, we describe a fast and cost-effective technique for isolating, culturing, and maintaining primary mouse astrocytes at ∼ 80% purity levels, which can be …
Formal UROP Report - University of Minnesota Twin Cities
Immunofluorescence staining of vinculin is a common technique that allows for quantification of focal adhesion size and quantity. A protocol for staining focal adhesions in vascular smooth …
