ssODN-mediated knock-in with CRISPR-Cas for large genomic …
2016年1月20日 · Here we show efficient gene KI in rats by combining CRISPR-Cas with single-stranded oligodeoxynucleotides (ssODNs). First, a 1-kb ssODN co-injected with guide RNA …
The ssODN is comprised of three parts: two homology arms sandwiching the region to be edited. The 5' homology arm (HA): this homology arm will encompass the ~50 nt upstream of the …
ssDNA和ssODNs - 知乎 - 知乎专栏
2023年7月6日 · ssODN ：寡核苷酸（single-stranded oligonucleotides） ssODNs包含一个24nt长的非同源中心区域， 以取代guide RNA ( gRNA ) 靶向的基因组DNA序列 ，同时在FKB12的3 …
ssODN Strategies for Large Fragment Insertion via CRISPR
2025年3月10日 · At the core of ssODN-mediated gene editing is homology-directed repair (HDR), a natural process that ssODNs exploit to introduce genetic changes. When a double-strand …
ssODN-mediated knock-in with CRISPR-Cas for large genomic ... - PubMed
2016年1月20日 · Here we show efficient gene KI in rats by combining CRISPR-Cas with single-stranded oligodeoxynucleotides (ssODNs). First, a 1-kb ssODN co-injected with guide RNA …
Highly efficient genome editing for single-base substitutions using ...
2019年3月18日 · To improve the efficiency of knock-in with single-stranded oligo DNA nucleotides (ssODNs), we have investigated optimal design of ssODNs in terms of the blocking mutation, …
Mechanistic and genetic basis of single-strand templated ... - Nature
2021年11月19日 · Single-stranded oligodeoxynucleotides (ssODNs) are widely used as DNA repair templates in CRISPR/Cas precision genome editing. However, the underlying …
Efficient ssODN-Mediated Targeting by Avoiding Cellular …
Combined with CRISPR-Cas9 technology and single-stranded oligodeoxynucleotides (ssODNs), specific single-nucleotide alterations can be introduced into a targeted genomic locus in …
Efficient In Vivo Introduction of Point Mutations Using ssODN and …
2020年7月14日 · We successfully applied the CRISPR-based white co-conversion strategy with a ssODN template, instead of the originally described dsDNA plasmid, to create genetically …
Efficient ssODN-Mediated Targeting by Avoiding Cellular ... - PubMed
2021年4月13日 · Combined with CRISPR-Cas9 technology and single-stranded oligodeoxynucleotides (ssODNs), specific single-nucleotide alterations can be introduced into …
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