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How to Reverse Pipette | Reverse Pipetting | Rainin - METTLER …
Reverse pipetting is handy when pipetting viscous, foaming, high vapor pressure or low surface tension liquids. A positive displacement pipette is an ideal instrument to precisely and …
Forward, Reverse, Repetitive, & Heterogeneous Sample Pipetting
Reverse pipetting avoids the risk of sample splash, foaming, or bubble formation. Press the operating button to the second stop. Dip the tip to a depth of 1cm, and slowly release the …
Reverse Technique: This technique is commonly used with air displacement pipettes, and is recommended for precisely pipetting small volumes. Reverse pipetting avoids the risk of …
10 Steps to Improve Pipetting Accuracy - Thermo Fisher Scientific
Standard (or forward) mode pipetting yields better accuracy and precision than reverse mode for all but viscous or volatile liquids. Reverse mode often results in over-delivery. Hence, it’s …
Reverse Pipetting - Sartorius
Reverse pipetting volatile liquids reduces dripping by eliminating the impact of any remaining evaporation on dispensing the total target volume. Any additional evaporating liquid escapes …
Forward and reverse pipetting are two basic pipetting modes that can be used with a mechanical pipette. In forward pipetting, the target volume is aspirated and dispensed, and a separate …
This application note demonstrates how the reverse pipetting technique can improve the repeatability of dispensing liquids that contain proteins. A pipette’s volume is commonly …
Reverse pipetting made easy - INTEGRA
Reverse pipetting is recommended for liquids that are very volatile or viscous, or that tend to foam up. Learn how to pipette them using the Reverse Pipet Mode.
Reduce errors by choosing a pipette/tip combination with the smallest possible air cushion. Immerse as little as possible. The hydrostatic pressure changes with the holding angle of the …
Increasing Precision When Pipetting Protein Samples: Assessing ...
2011年2月1日 · When dispensing small volumes of liquid-containing proteins, such as BSA, highly precise and repeatable resulting data can be obtained with the reverse pipetting technique. …
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