2023年11月1日 · In this study, we present a rapid and universal approach to establishing an all-in-one RPA-Cas12a method CORDSv2 based on rational balancing amplification and Cas12a cleavage, which achieves ultrasensitive detection of several targets, including SARS-CoV-2, ASFV, HPV16, and HPV18.

2025年3月15日 · The sequential integration of RPA with the highly sensitive Cas12a-dependent signal boosting significantly elevated the detection sensitivity for miRNA-21, enabling its identification at concentrations below 100 aM.

2023年11月1日 · In this study, we present a rapid and universal approach to establishing an all-in-one RPA-Cas12a method CORDSv2 based on rational balancing amplification and Cas12a cleavage, which achieves ultrasensitive detection of several targets, including SARS-CoV-2, ASFV, HPV16, and HPV18.

2024年8月1日 · We developed a one-pot RPA/CRISPR-Cas12a assay for nucleic acid detection using a photomodulated aptamer-based inhibitor. The incorporation of an aptamer with a PC linker allowed us to effectively block the cleavage activity of Cas12a RNP, which could be activated later via brief UV irradiation.

2024年4月23日 · The sensitive quantification is realized by utilizing dual PAM-free crRNAs for CRISPR/Cas12a recognition. The varied RPA primer concentration with stabilized CRISPR systems significantly affects the amplification efficiency and quantitative performances.

2025年1月31日 · In this study, we developed an advanced one-pot recombinase polymerase amplification (RPA)-CRISPR/Cas12a system, enhanced with glycerol and betaine, for ultrasensitive detection of the mecA gene.

2024年6月10日 · In this study, a dual-signal visualized detection platform with fluorescence assay and paper-based lateral flow assay (LFA) based on recombinase polymerase amplification (RPA), CRISPR/Cas12a system, and self-developed CRISPR nucleic acid test strips was constructed for enterotoxigenic B. cereus.

6 天之前 · Using this one-pot RT-RPA CRISPR/Cas12a detection system, all four serotypes of DENV (1 to 4) were successfully identified. In terms of specificity, the assay accurately detected DENV-infected positive samples without cross-reactivity with four other interfering viruses-infected samples (VSV, SeV, HSV-1 and IAV). Furthermore, we established a ...

3 天之前 · Integration of RPA with our CRISPR/Cas12a detection system would decrease the assay duration from 70 min to approximately 40 min—notably faster than standard CRISPR/Cas-based analyses requiring 50–300 min . Furthermore, RPA implementation would improve field-deployability by eliminating dependency on specialized thermal cycling equipment ...

2024年1月15日 · Clustered regularly interspaced short palindromic repeats (CRISPR)-based nucleic acid detection technology combined with recombinase polymerase amplification (RPA) allows for convenient, rapid, and highly sensitive nucleic acid detection.