Ribonuclease H (abbreviated RNase H or RNH) is a family of non-sequence-specific endonuclease enzymes that catalyze the cleavage of RNA in an RNA/DNA substrate via a hydrolytic mechanism. Members of the RNase H family can be found in nearly all organisms, from bacteria to archaea to eukaryotes .

Ribonuclease H (RNase H) is an endoribonuclease which specifically hydrolyzes the phosphodiester bonds of RNA which is hybridized to DNA. This enzyme does not digest single- or double-stranded DNA.

Ribonucleases H are enzymes that cleave the RNA of RNA/DNA hybrids that form during replication and repair and which could lead to DNA instability if they were not processed. There are two main types of RNases H, and at least one of them is present in most organisms.

RNase H is a ubiquitous enzyme that degrades the RNA strand of an RNA–DNA duplex. It has been identified in organisms as diverse as viruses and human cells (Crouch and Dirksen, 1985). At least two classes of RNase H have been identified in eukaryotic cells.

RNases H are a family of endonucleases that hydrolyze RNA residues in various nucleic acids. These enzymes are present in all branches of life, and their counterpart domains are also found in reverse transcriptases (RTs) from retroviruses and retroelements.

2019年12月1日 · RNases H are a family of endonucleases that hydrolyze RNA residues in various nucleic acids. These enzymes are present in all branches of life, and their counterpart domains are also found in reverse transcriptases (RTs) from retroviruses and retroelements.

The RNase H domains of M-MLV and HIV-1 reverse transcriptases are structurally very similar to the Escherichia coli and Bacillus halodurans RNases H, and to human RNase H1, and these similarities provide key insights concerning substrate recognition and catalysis by …

2024年7月5日 · Ribonuclease H (RNase H) is a critical enzyme found in various organisms, ranging from bacteria to humans. Its ability to selectively degrade the RNA strand in RNA-DNA hybrids makes it indispensable for numerous biological processes, including DNA …

Ribonuclease H-like (RNHL) superfamily, also called the retroviral integrase superfamily, groups together numerous enzymes involved in nucleic acid metabolism and implicated in many biological processes, including replication, homologous recombination, DNA repair, transposition and RNA interference.

Ribonuclease H* (RNase H, EC 3.1.26.4) is an endonuclease that specifically hydrolyzes an RNA hybridized to a complementary DNA to produce an oli-goribonucleotide with 5′-phosphate and 3′-hydroxyl groups (Fig. 1). It requires divalent cations, such as Mg 2+ and Mn 2+, for activity.