一、RNF8，全称 ring finger protein 8，是近年发现的一种 DNA损伤应答 蛋白。它包含 485 个氨基酸残基， 在人体组织中广泛表达， 是一种含有 FHA 及 RING 结构域的 E3 泛素连接酶 。

E3 ubiquitin-protein ligase RNF8 is an enzyme that in humans is encoded by the RNF8 gene. [5][6][7] RNF8 has activity both in immune system functions [8] and in DNA repair. The protein encoded by this gene contains a RING finger motif and an FHA domain.

Dec 25, 2024 · RNF8 (Ring Finger Protein 8) is a Protein Coding gene. Diseases associated with RNF8 include Riddle Syndrome and Xeroderma Pigmentosum, Variant Type. Among its related pathways are HDR through Homologous Recombination (HRR) or Single Strand Annealing (SSA) and DNA Double Strand Break Response.

RNF8 is the human orthologue of the yeast protein Dma1p; RNF8 is a novel DNA-damage-responsive protein that integrates protein phosphorylation and ubiquitylation signaling and plays a critical role in the cellular response to genotoxic stress. RNF8 ubiquitylates histones at DNA double-strand breaks and promotes assembly of repair proteins.

该基因编码的蛋白质包含一个无名指基序和一个 FHA 结构域。 这种蛋白质已被证明与几种 II 类泛素结合酶 (E2) 相互作用，包括 UBE2E1/UBCH6、UBE2E2 和 UBE2E3，并且可以在某些核蛋白的泛素化中充当泛素连接酶 (E3) 。 还已知这种蛋白质在 DNA 损伤反应中发挥作用，这种蛋白质的消耗会导致细胞生长抑制和细胞周期停滞。 可变剪接导致多个转录本变体。 [RefSeq 提供，2012 年 2 月] The protein encoded by this gene contains a RING finger motif and an FHA domain.

Jan 22, 2012 · Nature Structural & Molecular Biology - RNF8 and RNF168 are E3 ligases that promote the ubiquitination of histones at sites of double-strand breaks. Now RNF8 is shown to form mainly...

Nov 30, 2007 · RNF8 is a key player involved in the crosstalk between different protein modifications, with its FHA domain required for its localization to DNA-damage foci through a phosphorylation-dependent interaction and its E3 ligase catalytic domain required for the further accumulation of Rap80, BRCA1, and 53BP1 (Figure 7F). The interplay between these ...

During spermatogenesis, sequestered in the cytoplasm by PIWIL1: RNF8 is released following ubiquitination and degradation of PIWIL1. Showing features for mutagenesis, natural variant. Abolishes interaction with ATM-phosphorylated MDC1. Abolishes interaction with human herpesvirus 1 ICP0.

Mar 7, 2025 · The ubiquitination of histone H2A/H2AX, catalyzed by RNF8/RNF168, is a crucial step in the repair of DNA double-strand breaks (DSBs), playing a significant role in transmitting and amplifying DNA...

RNF8 is a key ubiquitin regulator in the DDR pathway. RNF8 translocates to DSBs via phospho-MDC1 and recruits BRCA1 and 53BP1 by ubiquitination of histone proteins and DDR-related factors (Fig. 1 F). At DSBs, RNF8 ubiquitinates substrates in the form of Lys63- or Lys48-linked poly-ubiquitination.