- Rapid amplification of cDNA ends
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See all on WikipediaRapid amplification of cDNA ends - Wikipedia
Rapid amplification of cDNA ends (RACE) is a technique used in molecular biology to obtain the full length sequence of an RNA transcript found within a cell. RACE results in the production of a cDNA copy of the RNA sequence of interest, produced through reverse transcription, followed by PCR amplification of … See more
The cDNA molecules generated by RACE can be sequenced using high-throughput sequencing technologies (also called, RACE-seq). High … See more
Wikipedia text under CC-BY-SA license 5´ RACE System for Rapid Amplification of cDNA Ends
Rapid Amplification of cDNA Ends (RACE) is a procedure for amplification of nucleic acid sequences from a messenger RNA template between a defined internal site and unknown …
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3´ RACE System for Rapid Amplification of cDNA Ends
Rapid Amplification of cDNA Ends (RACE) is a procedure for amplification of nucleic acid sequences from a messenger RNA template between a defined internal site and either the 3´ …
5′ end cDNA amplification using classic RACE - Nature
Dec 29, 2006 · RACE (rapid amplification of cDNA ends) PCR is useful for quickly obtaining full length cDNAs for mRNAs for which only part of the sequence is …
- Author: Elizabeth Scotto–Lavino, Guangwei Du, Michael A Frohman
- Publish Year: 2006
Simple and inexpensive three-step rapid amplification of cDNA 5′ …
Rapid amplification of cDNA 5′ ends (5′-RACE) is routinely used for the sequence analysis of the upstream noncoding regions of cellular mRNAs; however, it represents a tedious and cost …
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3′ End cDNA amplification using classic RACE - Nature
Jan 11, 2007 · In 3′ RACE, the poly (A) tail functions as a non-specific tag at the 3′ end of the mRNA. cDNA ends can be obtained in 1–3 days using this protocol. PCR can be used to …
3’ Rapid Amplification of cDNA Ends (3’ RACE) Using
We provide a detailed protocol of our 3’ RACE procedure comprising four major parts: Total RNA extraction, RNA amount determination and quality control, the RACE procedure itself, and …
cDNA amplification kits for 5' and 3' RACE - Takara Bio
Rapid amplification of cDNA ends (RACE) is a technique used to obtain the full-length sequences of RNA transcripts. RACE can provide the sequence of an RNA transcript from a short, known sequence within the transcript all the way to the …
Improved rapid amplification of cDNA ends (RACE) for mapping …
Dec 1, 2005 · Rapid amplification of cDNA ends (RACE) is a powerful PCR-based technique for determination of RNA terminal sequences. However, most of the RACE methods reported in …
1 Overview of the 5' RACE System Procedure..... 3 2 Sequences of the 5' RACE Primers ..... 4 3 Effect of GSP1 on 5' RACE Efficiency ..... 7
