RNA sequencing (RNA-Seq) technology enables rapid profiling and deep investigation of the transcriptome, for any species. This approach offers a number of advantages compared to microarray analysis, a legacy technology often used in gene expression studies. Benefits of RNA-Seq vs microarrays.

These studies overall indicate that: (1) a significantly larger number of DEGs and affected pathways can be detected with RNA-Seq compared to microarrays; (2) RNA-Seq has a wider dynamic range than microarrays; and (3) a reasonable concordance of DEGs (50–60%) exists between the two platforms.

2024年7月5日 · Both RNA-Seq and microarrays are used in functional genomics to understand how genes are regulated and how they interact with each other. RNA-Seq’s ability to detect novel transcripts and provide quantitative data makes it particularly valuable in this field.

6 天之前 · Microarray technology is a general laboratory approach that involves binding an array of thousands to millions of known nucleic acid fragments to a solid surface, referred to as a “chip.” The chip is then bathed with DNA or RNA isolated from a study sample (such as cells or tissue).

The development of microarray technology has revolutionized RNA and deoxyribonucleic acid (DNA) research. In contrast with traditional biological assays, microarrays allow the simultaneous measurement of tens of thousands of messenger RNA (mRNA) transcripts for gene expression or of genomic DNA frag …

Microarray and RNA sequencing are both powerful techniques used in molecular biology to study gene expression. However, they differ in their methodology and applications. Microarray measures gene expression by hybridizing labeled cDNA or RNA samples to a chip containing thousands of known DNA sequences.

2023年10月3日 · microarrays are a powerful tool for analyzing gene expression levels in living organisms. They are composed of thousands of individual DNA fragments that are immobilized on a solid support such as glass.

2019年1月21日 · RNA-Seq technology has emerged as an attractive alternative to traditional microarray platforms for conducting transcriptional profiling. The objective of this work was to compare both transcriptomic platforms to determine whether RNA-Seq offered significant advantages over microarrays for toxicogenomic studies.

Among its diverse applications, RNA microarray stands out as a pivotal method for probing RNA expression levels with precision and efficiency. Here, we delve into the workings of the microarray, shedding light on its methodology and transformative potential.

MicroRNAs (miRNAs) are regulatory noncoding RNA molecules ∼20–23 nucleotides (nt) long, generated by two cleavage events mainly from RNA Pol II primary transcripts (pri-miRNAs) via a ∼70-nt imperfect stem–loop intermediate (pre-miRNA).