
pYES2 Sequence and Map - SnapGene
Download pYES2.dna file. Download Plasmid Open in SnapGene. SnapGene. SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures. …
pYES2 Yeast Expression Vector - Thermo Fisher Scientific
The pYES2 vector is designed for native expression of your protein of interest in S. cerevisiae. It contains the URA3 gene for selection in yeast and 2 origin for high-copy maintenance.
pYES2 NT C Sequence and Map - SnapGene
High-copy episomal vector for galactose-inducible expression of N-terminally tagged proteins in S. cerevisiae. For other reading frames, use pYES2/NT A or pYES2/NT B.
in an expression plasmid (i.e. pYES2/NT), the presence of the 2μ origin allows the plasmid to be episomally maintained and replicated at high copy number (generally 10–40 copies per cell).
1.将目的蛋白克隆到pYES2载体(多克隆位点请参照图谱序列)。 2.将重组载体转化到大肠杆菌感受态细胞中(参照TOP10F’感受态细胞使用说明书)。 3.用含氨苄(50-100ug/mL)的LB平 …
The parental pYES2 vector (Figure 3) is utilized for native expression of your protein of interest. It contains the URA3 selection marker for selection in yeast. The pYES2/NT vector (Figure 4), …
Introduction pYES2 is a 5.9 kb vector designed for inducible expression of recombinant proteins in Saccharomyces cerevisiae . Features of the vector allow easy cloning of
pYES2 载体图谱和序列 - 纽普生物
The pYES2 vector is an episomal high-copy plasmid designed for galactose-inducible protein expression in Saccharomyces cerevisiae.
pYES2质粒图谱、载体图谱、序列、价格、抗性、测序引物、大小 …
pYES2的是一个5.9 kb的载体,设计用来在酿酒酵母(Saccharomyces cerevisiae)中诱导表达重组蛋白。 载体的特点在于基因插入载体的构建简单,以及能够使用原养型尿嘧啶进行转化株 …
pYES2/CT, pYES3/CT, and pYC2/CT are 6.0 kb, 5.9 kb, and 4.6 kb vectors, respectively, designed for inducible expression of recombinant proteins in Saccharomyces cerevisiae. …