Bacterial expression vector that encodes a thiol-cleavable C-terminal intein followed by a chitin-binding domain. Sequence Author: New England Biolabs. Explore Over 2.7k Plasmids: Basic Cloning Vectors | More Plasmid Sets. Sticky ends from different BlpI sites may not be compatible.

pTXB1 is an E. coli expression vector designed for the in-frame insertion of a target gene into the polylinker upstream of the Mxe intein/chitin binding domain. The fusion protein is bound to chitin beads and the thiol-induced cleavage activity of the intein releases the target protein.

Plasmid pTXB1-Tn5 from Dr. Rickard Sandberg's lab contains the insert tnpA from pBam1 and is published in Genome Res. 2014 Jul 30. pii: gr.177881.114. This plasmid is available through Addgene.

pTXB1 is an E. coli plasmid cloning vector designed for recom-binant protein expression, purification, and ligation using the IMPACTTM Kit (NEB #E6901) (1,2). It contains the pMB1 origin of replication from pBR322 and is maintained at a similar copy number to pBR322; in addition, pTXB1 also contains an M13 origin of replication.

Provide pTXB1 vector/plasmid map, full length sequence, antibiotic resistance, size and other information

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Plasmid pTXB1-TnY from Dr. Peter Smibert's lab contains the insert TnY and is published in Nat Biotechnol. 2021 Apr 29. pii: 10.1038/s41587-021-00902-x. doi: 10.1038/s41587-021-00902-x. This plasmid is available through Addgene.

2022年12月19日 · The pTXB1-nbTn5 vector was transformed into BL21(DE3)-competent Escherichia coli cells (NEB, C2527), and nb–Tn5 was produced via intein purification with an affinity chitin-binding tag 24.

pTXB1 is an E. coli plasmid cloning vector designed for recom-binant protein expression, purification, and ligation using the IMPACTTM Kit (NEB #E6901) (1, 2). It contains the pMB1 origin of replication from pBR322 and is maintained at a similar copy number to pBR322; in addition, pTXB1 also contains an M13 origin of replication.

pTXB1 is an E. coli plasmid cloning vector designed for recom-binant protein expression, purification, and ligation using the IMPACTTM Kit (NEB #E6901) (1, 2). It contains the pMB1 origin of replication from pBR322 and is maintained at a similar copy number to pBR322; in addition, pTXB1 also contains an M13 origin of replication.