Dec 6, 2007 · Here, we show that proteins fused to the human cytomegalovirus tegument protein pp65 were bound with higher efficiency to DCs than those fused to the described PTDs TatPTD and Penetratin....

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser536 of human NF-κB p65. Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in …

Functional genomics revealed a role for the major cytomegalovirus structural protein, pp65 (ppUL83), in counteracting this response. The underlying mechanism involves a differential impact of this structural protein on the regulation of interferon response factor 3 (IRF-3).

To comprehensively analyze CD8 + T cell responses against the CMV pp65 antigen restricted by a single HLA class I allotype, we conducted ELISPOT assays using an artificial antigen-presenting cell (aAPC) expressing both the pp65 antigen and each HLA class I allotype present in a donor.

Here we describe a pp65‐derived peptide (pp65 340–355, RQYDPVAALFFFDIDL: RQY16‐mer), characterized by peculiar features. First, RQY‐16mer is able to stimulate HCMV pp65 specific responses in both CD4 + and CD8 + T cells, restricted by a wide range of HLA class I …

Jan 17, 2012 · Pp65 is the most abundant tegument protein and the major constituent of extracellular virus particles . However, pp65 is not essential for the production of new infectious virus particles as evidenced in strains that lack the pp65 gene which can still replicate in culture.

Jun 15, 2020 · Identification of the major late human cytomegalovirus matrix protein pp65 as a target antigen for CD8+ virus-specific cytotoxic T lymphocytes. J Med Virol 43, 103–110 (1994).

This article focuses on HCMV tegument pp65 by reviewing its mechanisms of action in favoring virus evasion from the host innate immune response. Keywords: Antiviral defense; DNA sensors; Human cytomegalovirus (HCMV); Intrinsic immunity; Restriction factors; Tegument proteins; Viral escape mechanisms; pp65.

Jan 18, 2016 · The human cytotoxic T-lymphocyte (CTL) response to cytomegalovirus is dominated by structural protein pp65: frequency, specificity and T-cell receptor usage of pp65-specific CTL. J Virol 70, 7569...

Aug 18, 2023 · Data indicate that cytomegalovirus pp65-specific T cells lyse autologous, primary glioblastoma (GBM) tumor cells in an antigen-specific manner. The results are concordant with a model of phosphoprotein 65 serving as an optional scaffold protein that facilitates protein upload into the outer tegument of human cytomegalovirus particles.